基于DNA适配体的可卡因传感器通过热处理改善其电化学响应

Improvement of Electrochemical Response of Cocaine Sensors Based on DNA Aptamer by Heat Treatment.

作者信息

Arimoto Satoshi, Shimono Ken, Yasukawa Tomoyuki, Mizutani Fumio, Yoshioka Toshihiko

机构信息

Advanced Research Division, Bio Research Department, Panasonic Corporation.

出版信息

Anal Sci. 2016;32(4):469-72. doi: 10.2116/analsci.32.469.

DOI:10.2116/analsci.32.469

PMID:27063722

Abstract

We report on a biosensor for cocaine based on the conformation change of DNA aptamer by capturing the cocaine molecules. The oxidation current of ferrocene conjugated on the terminal end of aptamer immobilized on an Au electrode increased with increasing cocaine concentration. The sensor response has been improved by a simple heat treatment after immobilization, since the aggregates of DNA aptamer generated during the immobilization step could be dissociated and rearranged on the electrode.

摘要

我们报道了一种基于DNA适配体构象变化来捕获可卡因分子的可卡因生物传感器。固定在金电极上的适配体末端共轭的二茂铁的氧化电流随可卡因浓度的增加而增大。固定后通过简单的热处理提高了传感器响应，因为固定步骤中产生的DNA适配体聚集体可在电极上解离并重新排列。