急性小鼠脑片中大麻素介导的突触调制的电生理测量
Electrophysiological Measurement of Cannabinoid-Mediated Synaptic Modulation in Acute Mouse Brain Slices.
作者信息
Báldi Rita, Ghosh Dipanwita, Grueter Brad A, Patel Sachin
机构信息
Department of Psychiatry, Vanderbilt University Medical Center, Nashville, Tennessee.
Department of Anesthesiology, Vanderbilt University Medical Center, Nashville, Tennessee.
出版信息
Curr Protoc Neurosci. 2016 Apr 8;75:6.29.1-6.29.19. doi: 10.1002/cpns.8.
Abstract
Endocannabinoids (eCBs) are a class of bioactive lipids that mediate retrograde synaptic modulation at central and peripheral synapses. The highly lipophilic nature of eCBs and the pharmacological tools available to interrogate this system require unique methodological consideration, especially when applied to ex vivo systems such as electrophysiological analysis in acute brain slices. This unit provides protocols for measuring cannabinoid and eCB-mediated synaptic signaling in mouse brain slices, including analysis of short-term, long-term, and tonic eCB signaling modes, and the unique considerations for working with eCBs and TRPV1/cannabinoid ligands in acute brain slices.
摘要
内源性大麻素（eCBs）是一类生物活性脂质，可介导中枢和外周突触的逆行性突触调节。eCBs的高度亲脂性以及用于研究该系统的药理学工具需要独特的方法学考量，尤其是在应用于离体系统时，如急性脑片的电生理分析。本单元提供了在小鼠脑片中测量大麻素和eCB介导的突触信号传导的方案，包括短期、长期和紧张性eCB信号传导模式的分析，以及在急性脑片中使用eCBs和TRPV1/大麻素配体的独特注意事项。
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