从辣椒茄中提取的具有抗高血压潜力的证据。在自发性高血压大鼠中。
Evidences of antihypertensive potential of extract from Solanum capsicoides All. in spontaneously hypertensive rats.
机构信息
Laboratório de Fisiologia e Farmacologia Endócrina e Cardiovascular, Departamento de Biorregulação, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Avenida Reitor Miguel Calmon, Vale do Canela, BA 40110-902 Salvador, Brazil.
Laboratório de Neurociências, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Avenida Reitor Miguel Calmon,Vale do Canela, BA 40110-902, Salvador, Brazil.
出版信息
Phytomedicine. 2016 May 15;23(5):498-508. doi: 10.1016/j.phymed.2016.02.020. Epub 2016 Mar 5.
BACKGROUND
Solanum capsicoides All. is morphologically similar to Solanum sisymbriifolium Lam. which is used in folk medicine in South America for antihypertensive and diuretics purposes. This similarity has led to species identification errors, which therefore may result in errors by patients.
PURPOSE
To evaluate the antihypertensive and diuretics potential of the methanol extract from Solanum capsicoides All. (MeOH-Sc), in vitro and in vivo, in spontaneously hypertensive rats (SHR).
METHODS
Initial experiments were performed in rat mesenteric artery to evaluate the in vitro vascular effect of MeOH-Sc and its fractions, in addition to the mechanisms involved during the observed effect. Mean arterial pressure (MAP) and heart rate (HR) were recorded in non-anesthetised hypertensive and normotensive rats. In another set of experiments, MeOH-Sc was administered for 21 consecutive days. Daily body weight measurements were conducted and MAP, HR and urinary volume were measured every 5 days. The mesenteric artery from treated animals was tested for phenylephrine and sodium nitroprussiate (SNP) sensitivity.
RESULTS
Initially, MeOH-Sc and fractions relaxed phenylephrine-induced contractions in mesenteric artery rings. The vasorelaxant effect was not changed in the presence of a blocker of eNOS (L-NAME) in rings with an intact endothelium. In denuded-endothelium rings, the vasorelaxant response was significantly reduced in the presence of a cAMP inhibitor (SQ 22536 10 µM) in SHR but not in Wistar Kyoto rats (WKY). However, in the presence of a cGMP inhibitor (ODQ 10 µM), a curve shift to the right was observed in WKY animals, but not in SHR. Intravenous bolus injections of MeOH-Sc into non-anesthetised SHR and WKY, induced hypotension that was associated with an increase in HR. A significant antihypertensive effect was observed in animals that received MeOH-Sc orally for 21 days, which also prevented the development of cardiac hypertrophy. Urine volume from animals treated with MeOH-Sc significantly increased. Finally, MeOH-Sc induced beneficial changes in vascular responsiveness.
CONCLUSION
MeOH-Sc has a potential antihypertensive effect in SHR.
背景
龙葵（Solanum capsicoides All.）在形态上与南美民间用于治疗高血压和利尿的茄属植物（Solanum sisymbriifolium Lam.）相似。这种相似性导致了物种鉴定错误，因此可能导致患者的用药错误。
目的
评估甲醇提取物（MeOH-Sc）对自发性高血压大鼠（SHR）的体外和体内降压和利尿作用。
方法
在大鼠肠系膜动脉中进行初步实验，以评估 MeOH-Sc 及其馏分的体外血管作用，以及观察到的作用过程中的相关机制。在非麻醉的高血压和正常血压大鼠中记录平均动脉压（MAP）和心率（HR）。在另一组实验中，连续 21 天给予 MeOH-Sc。每天测量体重，每隔 5 天测量 MAP、HR 和尿量。用酚妥拉明和硝普钠（SNP）检测治疗动物的肠系膜动脉敏感性。
结果
最初，MeOH-Sc 和馏分舒张了肠系膜动脉环中由苯肾上腺素引起的收缩。在完整内皮的环中，存在内皮型一氧化氮合酶（eNOS）抑制剂（L-NAME）时，血管舒张作用没有改变。在内皮剥脱的环中，在 SHR 中，环磷酸鸟苷（cGMP）抑制剂（ODQ 10 µM）存在时，血管舒张反应明显降低，但在 Wistar Kyoto 大鼠（WKY）中则没有。然而，在 WKY 动物中，存在环磷酸鸟苷（cGMP）抑制剂（ODQ 10 µM）时，曲线向右移位，但在 SHR 中则没有。静脉注射 MeOH-Sc 到非麻醉的 SHR 和 WKY 中，引起低血压，同时伴有心率增加。在接受 MeOH-Sc 口服治疗 21 天的动物中观察到显著的降压作用，这也防止了心脏肥大的发展。用 MeOH-Sc 治疗的动物的尿量显著增加。最后，MeOH-Sc 诱导了血管反应性的有益变化。
结论
MeOH-Sc 对 SHR 具有潜在的降压作用。
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