Hammann Simon, Vetter Walter
Institute of Food Chemistry (170b), University of Hohenheim , Garbenstraße 28, D-70599 Stuttgart, Germany.
J Agric Food Chem. 2016 May 4;64(17):3437-44. doi: 10.1021/acs.jafc.6b00383. Epub 2016 Apr 19.
Ergosterol is the major sterol in button mushrooms (Agaricus bisporus) and can occur as free alcohol or esterified with fatty acids (ergosteryl esters). In this study, gas chromatography with mass spectrometry in the selected ion monitoring mode (GC/MS-SIM) was used to determine ergosterol and ergosteryl esters as well as other sterols and steryl esters in button mushrooms. Different quality control measures were established and sample preparation procedures were compared to prevent the formation of artifacts and the degradation of ergosteryl esters. The final method was then used for the determination of ergosterol (443 ± 44 mg/100 g dry matter (d.m.)) and esterified ergosterol (12 ± 6 mg/100 g d.m.) in button mushroom samples (n = 4). While the free sterol fraction was vastly dominated by ergosterol (∼90% of five sterols in total), the steryl ester fraction was more diversified (nine sterols in total, ergosterol ∼55%) and consisted primarily of linoleic acid esters.
麦角固醇是双孢蘑菇（Agaricus bisporus）中的主要固醇，它可以以游离醇的形式存在，也可以与脂肪酸酯化形成麦角固醇酯。在本研究中，采用选择离子监测模式的气相色谱-质谱联用（GC/MS-SIM）来测定双孢蘑菇中的麦角固醇、麦角固醇酯以及其他固醇和固醇酯。建立了不同的质量控制措施，并比较了样品制备程序，以防止假象的形成和麦角固醇酯的降解。最终该方法用于测定双孢蘑菇样品（n = 4）中的麦角固醇（443 ± 44 mg/100 g干物质（d.m.））和酯化麦角固醇（12 ± 6 mg/100 g d.m.）。虽然游离固醇部分主要由麦角固醇主导（占五种固醇总量的约90%），但固醇酯部分则更加多样化（总共九种固醇，麦角固醇约占55%），且主要由亚油酸酯组成。
