Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil.
D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil.
Science. 2016 May 13;352(6287):816-8. doi: 10.1126/science.aaf6116. Epub 2016 Apr 10.
Since the emergence of Zika virus (ZIKV), reports of microcephaly have increased considerably in Brazil; however, causality between the viral epidemic and malformations in fetal brains needs further confirmation. We examined the effects of ZIKV infection in human neural stem cells growing as neurospheres and brain organoids. Using immunocytochemistry and electron microscopy, we showed that ZIKV targets human brain cells, reducing their viability and growth as neurospheres and brain organoids. These results suggest that ZIKV abrogates neurogenesis during human brain development.
自寨卡病毒（ZIKV）出现以来，巴西报告的小头畸形病例数量大幅增加；然而，病毒流行与胎儿大脑畸形之间的因果关系仍需进一步证实。我们研究了 ZIKV 感染对作为神经球和脑类器官生长的人神经干细胞的影响。通过免疫细胞化学和电子显微镜，我们发现 ZIKV 可靶向人脑细胞，降低其作为神经球和脑类器官的活力和生长。这些结果表明，ZIKV 可破坏人类大脑发育过程中的神经发生。
