肠道耐药组在生命的最初几个月中高度动态变化。
The gut resistome is highly dynamic during the first months of life.
作者信息
von Wintersdorff Christian J H, Wolffs Petra F G, Savelkoul Paul H M, Nijsen Rianne R R, Lau Susanne, Gerhold Kerstin, Hamelmann Eckard, Penders John
机构信息
Department of Medical Microbiology, NUTRIM School for Nutrition & Translational Research in Metabolism, Maastricht University Medical Center+, Maastricht, The Netherlands.
Department of Medical Microbiology, Caphri School for Public Health & Primary Care, Maastricht University Medical Center+, Maastricht, The Netherlands.
出版信息
Future Microbiol. 2016;11(4):501-10. doi: 10.2217/fmb.15.154. Epub 2016 Apr 11.
AIM
We investigated the longitudinal development of several antibiotic resistance genes (ARGs) of the infant gut resistome during the first months after birth.
MATERIALS & METHODS: Fecal samples from 120 infants collected at the ages of 5, 13 and 31 weeks were analyzed and subjected to qPCR for the detection of several ARGs.
RESULTS
The prevalence of ARGs significantly increased for ermB, tetM and tetQ, while it decreased for aac(6')-aph(2'). Birth mode and breastfeeding significantly affected tetQ prevalence. Correlations to bacterial taxa suggest that fluctuations in some ARGs are (partly) attributed to shifts in bacteroides colonization rates.
CONCLUSION
Acquisition of ARGs in the gut microbiota occurs shortly after birth and resistome composition fluctuates over the course of several months, reflecting changes in microbial community structure.
目的
我们研究了出生后头几个月婴儿肠道耐药组中几种抗生素抗性基因（ARGs）的纵向发育情况。
材料与方法
分析了120名婴儿在5、13和31周龄时采集的粪便样本，并进行qPCR检测几种ARGs。
结果
ermB、tetM和tetQ的ARGs流行率显著增加，而aac(6')-aph(2')的流行率下降。分娩方式和母乳喂养显著影响tetQ的流行率。与细菌类群的相关性表明，一些ARGs的波动（部分）归因于拟杆菌定植率的变化。
结论
肠道微生物群中的ARGs在出生后不久就会获得，耐药组组成在几个月的过程中波动，反映了微生物群落结构的变化。
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