Zhang Haoqiang, Liu Zhenkun, Chen Hui, Tang Ming
College of Forestry, Northwest A&F University, Yangling, Shaanxi, 712100, PR China.
PLoS One. 2016 Apr 11;11(4):e0153378. doi: 10.1371/journal.pone.0153378. eCollection 2016.
Robinia pseudoacacia L. (black locust) is a widely planted tree species on Loess Plateau for revegetation. Due to its symbiosis forming capability with arbuscular mycorrhizal (AM) fungi, we explored the influence of arbuscular mycorrhizal fungi on plant biomass, root morphology, root tensile strength and soil aggregate stability in a pot experiment. We inoculated R. pseudoacacia with/without AM fungus (Rhizophagus irregularis or Glomus versiforme), and measured root colonization, plant growth, root morphological characters, root tensile force and tensile strength, and parameters for soil aggregate stability at twelve weeks after inoculation. AM fungi colonized more than 70% plant root, significantly improved plant growth. Meanwhile, AM fungi elevated root morphological parameters, root tensile force, root tensile strength, Glomalin-related soil protein (GRSP) content in soil, and parameters for soil aggregate stability such as water stable aggregate (WSA), mean weight diameter (MWD) and geometric mean diameter (GMD). Root length was highly correlated with WSA, MWD and GMD, while hyphae length was highly correlated with GRSP content. The improved R. pseudoacacia growth, root tensile strength and soil aggregate stability indicated that AM fungi could accelerate soil fixation and stabilization with R. pseudoacacia, and its function in revegetation on Loess Plateau deserves more attention.
刺槐是黄土高原广泛种植用于植被恢复的树种。由于其与丛枝菌根（AM）真菌形成共生关系的能力，我们在盆栽试验中探究了丛枝菌根真菌对植物生物量、根系形态、根系抗拉强度和土壤团聚体稳定性的影响。我们对接种/未接种AM真菌（不规则球囊霉或多样硬囊霉）的刺槐进行了处理，并在接种后12周测量了根定殖、植物生长、根系形态特征、根系拉力和抗拉强度以及土壤团聚体稳定性参数。AM真菌定殖于70%以上的植物根系，显著促进了植物生长。同时，AM真菌提高了根系形态参数、根系拉力、根系抗拉强度、土壤中球囊霉素相关土壤蛋白（GRSP）含量以及土壤团聚体稳定性参数，如水稳性团聚体（WSA）、平均重量直径（MWD）和几何平均直径（GMD）。根长与WSA、MWD和GMD高度相关，而菌丝长度与GRSP含量高度相关。刺槐生长、根系抗拉强度和土壤团聚体稳定性的改善表明，AM真菌可以加速刺槐对土壤的固定和稳定，其在黄土高原植被恢复中的作用值得更多关注。
