Liu W, Choi B R, Bak Y O, Zhang L T, Zhou L X, Huang Y R, Zhao C, Park J K
Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University and Shanghai Institute of Andrology Departments of Urology Shanghai China.
Chonbuk National University Hospital Departments of Urology Jeonju South Korea.
Cell Mol Biol (Noisy-le-grand). 2016 Mar 31;62(3):115-9.
To evaluate the effect of Schisandrol A on rabbit corpus cavernosum smooth muscle and elucidate the potential mechanism. Penises were obtained from healthy male New Zealand White rabbits (2.5-3.0 kg). The pre-contracted penis with phenylephrine (Phe, 10 µM) was treated with accumulative concentrations of Schisandrol A (10-7, 10-6, 10-5 and 10-4 M). The change in intracavernosum pressure (ICP) and tension was recorded, cyclic nucleotides in the cavernosum tissue were measured by radioimmunoassay, mRNA level and expression of endothelial nitric oxide synthase (eNOS) and neuronal NOS (nNOS) were measured by real time PCR and western blot respectively. The corpus cavernosum smooth muscle relaxation induced by Schisandrol A was in a dose-dependent manner. Pre-treatment with NOS inhibitor (Nω nitro-L-arginine-methyl ester, L-NAME) or guanylyl cyclase inhibitor (1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one, ODQ) significantly diminished the relaxation. The cyclic guanosine monophosphate (cGMP) level was significantly increased in the cavernosum tissue. Real time PCR and western blot showed the mRNA level and expression of eNOS and nNOS was also upregulated. Schisandrol A relaxes the cavernosum smooth muscle by activating NO-cGMP signaling pathway. It may be a new promising treatment for erectile dysfunction and cardiovascular disease.
评估五味子醇甲对兔海绵体平滑肌的作用并阐明其潜在机制。阴茎取自健康雄性新西兰白兔（体重2.5 - 3.0千克）。用苯肾上腺素（Phe，10 μM）预收缩的阴茎分别用累积浓度的五味子醇甲（10-7、10-6、10-5和10-4 M）处理。记录海绵体内压（ICP）和张力的变化，用放射免疫分析法测定海绵体组织中的环核苷酸，分别用实时PCR和蛋白质免疫印迹法测定内皮型一氧化氮合酶（eNOS）和神经元型一氧化氮合酶（nNOS）的mRNA水平和表达。五味子醇甲诱导的海绵体平滑肌松弛呈剂量依赖性。用一氧化氮合酶抑制剂（Nω-硝基-L-精氨酸甲酯，L-NAME）或鸟苷酸环化酶抑制剂（1H-(1,2,4)恶二唑并(4,3-a)喹喔啉-1-酮，ODQ）预处理可显著减弱这种松弛作用。海绵体组织中环磷酸鸟苷（cGMP）水平显著升高。实时PCR和蛋白质免疫印迹显示eNOS和nNOS的mRNA水平和表达也上调。五味子醇甲通过激活NO-cGMP信号通路使海绵体平滑肌松弛。它可能是治疗勃起功能障碍和心血管疾病的一种新的有前景的疗法。
