血管加压素可兴奋位于豚鼠脑干蜗神经背核的神经元。

Vasopressin excites neurones located in the dorsal cochlear nucleus of the guinea-pig brainstem.

作者信息

Charpak S, Dubois-Dauphin M, Raggenbass M, Dreifuss J J

机构信息

Department of Physiology, University Medical Center, Geneva, Switzerland.

出版信息

Brain Res. 1989 Mar 27;483(1):164-9. doi: 10.1016/0006-8993(89)90049-8.

DOI:10.1016/0006-8993(89)90049-8

PMID:2706504

Abstract

The effect of arginine vasopressin (AVP) on neurones in the dorsal cochlear nucleus (DCN) of young guinea-pigs of either sex was investigated in brainstem slices. Most impaled neurones fired in a regular manner, either spontaneously or following a depolarizing current injection. AVP, at concentrations of 10-1000 nM, excited 19/19 neurones from male and 16/19 neurones from female animals. This effect of AVP was concentration-dependent and could be mimicked by the V1 agonist [Phe2,Orn8]VT. Oxytocin was less potent than AVP and a selective V2 agonist, deamino-DAVP, was without effect. Thus, a class of DCN neurones is probably endowed with functional V1 vasopressin receptors. By making use of an antibody raised against the vasopressin-related glycopeptide, dense AVP-like immunoreactivity was found in the DCN of young animals of either sex.

摘要

在脑干切片中研究了精氨酸加压素（AVP）对幼年豚鼠（无论雌雄）蜗神经背核（DCN）神经元的影响。大多数被穿刺的神经元以规则的方式放电，要么自发放电，要么在注入去极化电流后放电。浓度为10 - 1000 nM的AVP使19只雄性动物的神经元中的19个以及19只雌性动物的神经元中的16个兴奋。AVP的这种作用具有浓度依赖性，并且可被V1激动剂[Phe2，Orn8]VT模拟。催产素的效力低于AVP，而选择性V2激动剂去氨加压素则无作用。因此，一类DCN神经元可能具有功能性V1加压素受体。通过使用针对加压素相关糖肽产生的抗体，在幼年豚鼠（无论雌雄）的DCN中发现了密集的AVP样免疫反应性。