Moran K, de Lange C F M, Ferket P, Fellner V, Wilcock P, van Heugten E
J Anim Sci. 2016 Mar;94(3):1031-40. doi: 10.2527/jas.2015-9855.
This study evaluated the effect of xylanase supplementation (with or without), feeding method (dry or liquid), and feedstuff (corn distiller's dried grains with solubles [DDGS] or wheat middlings) on apparent total tract digestibility (ATTD) and apparent ileal digestibility (AID) of GE and nutrients, intestinal morphology, ileal and cecal pH, and VFA concentrations. Sixty-four growing pigs (25.87 ± 0.38kg initial BW) were blocked by BW and sex and randomly assigned to 8 dietary treatments. Within each feedstuff, diets were fed either liquid or dry, without or with xylanase (24,000 birch xylan units/kg feed), for 16 d. Diets contained 3.32 and 3.19 Mcal/kg ME for DDGS- and wheat middlings-based diets, respectively. Pigs were fed restricted at 3 times maintenance ME requirements. Liquid diets were prepared by steeping DDGS or wheat middlings with water (1:3, wt/vol) with or without xylanase for 24 h followed by mixing with a basal ingredient mixture and water to achieve a final ratio of 1:2.5 (wt/vol). During steeping of wheat middlings, some fiber degradation occurred. When xylanase was added in dry wheat middlings diets, AID of GE ( < 0.10) and NDF ( < 0.05) increased compared with dry wheat middlings diets without xylanase (64.50 vs. 54.67% and 52.88 vs. 31.69%, respectively), but supplementation of xylanase did not impact AID of GE and NDF when liquid wheat middlings diets were fed. Xylanase in liquid DDGS diets increased ( < 0.05) the AID of NDF compared with liquid DDGS diets without xylanase, but xylanase did not affect AID of NDF in dry DDGS diets. Xylanase in wheat middlings diets improved ( < 0.05) ATTD of GE and N compared with wheat middlings diets without xylanase (80.37 vs. 78.07% and 80.23 vs. 77.94%, respectively). However, there was no effect of xylanase in DDGS diets. Pigs fed DDGS diets had greater concentrations of butyrate in the cecum ( = 0.001) than pigs fed wheat middlings diets (27.6 vs. 20.4 mmol/L). Pigs fed DDGS diets with xylanase had deeper crypts ( < 0.05) in the jejunum than pigs fed DDGS diets without xylanase (98.20 vs. 86.16 μm), but xylanase had no effect in pigs fed wheat middlings diets. Results suggest that liquid feeding and xylanase supplementation had limited potential to enhance nutrient digestibility in pigs fed DDGS-based diets. However, xylanase supplementation in dry wheat middlings-based diets improved the AID of NDF and ATTD of GE and N, but liquid feeding as pretreatment did not further enhance the nutritional value of wheat middlings-based diets.
本研究评估了木聚糖酶添加(添加或不添加)、饲喂方式(干喂或湿喂)和饲料原料(玉米干酒糟及其可溶物[DDGS]或小麦麸)对总能(GE)和养分的表观全肠道消化率(ATTD)、表观回肠消化率(AID)、肠道形态、回肠和盲肠pH值以及挥发性脂肪酸(VFA)浓度的影响。64头生长猪(初始体重25.87±0.38kg)按体重和性别进行分组,并随机分配到8种日粮处理中。在每种饲料原料中,日粮分别采用湿喂或干喂方式,不添加或添加木聚糖酶(24,000桦木木聚糖酶单位/kg饲料),持续16天。以DDGS和小麦麸为基础的日粮的代谢能(ME)分别为3.32和3.19Mcal/kg。按维持能量需求的3倍对猪进行限饲。湿日粮的制备方法是将DDGS或小麦麸与水(1:3,重量/体积)浸泡24小时,添加或不添加木聚糖酶,然后与基础成分混合物和水混合,最终比例达到1:2.5(重量/体积)。在浸泡小麦麸的过程中,发生了一些纤维降解。当在干小麦麸日粮中添加木聚糖酶时,与不添加木聚糖酶的干小麦麸日粮相比,GE的AID(<0.10)和中性洗涤纤维(NDF)的AID(<0.05)有所增加(分别为64.50%对54.67%和52.88%对31.69%),但在饲喂湿小麦麸日粮时,添加木聚糖酶对GE和NDF的AID没有影响。与不添加木聚糖酶的湿DDGS日粮相比,湿DDGS日粮中的木聚糖酶使NDF的AID增加(<0.05),但木聚糖酶对干DDGS日粮中NDF的AID没有影响。与不添加木聚糖酶的小麦麸日粮相比,小麦麸日粮中的木聚糖酶提高了(<0.05)GE和氮(N)的ATTD(分别为80.37%对78.07%和80.23%对77.94%)。然而,DDGS日粮中的木聚糖酶没有效果。饲喂DDGS日粮的猪盲肠中的丁酸浓度(P = 0.001)高于饲喂小麦麸日粮的猪(27.6对20.4mmol/L)。与不添加木聚糖酶的DDGS日粮相比,饲喂添加木聚糖酶的DDGS日粮的猪空肠隐窝更深(<0.05)(98.20对86.16μm),但木聚糖酶对饲喂小麦麸日粮的猪没有影响。结果表明,湿喂和添加木聚糖酶对提高以DDGS为基础日粮的猪的养分消化率的潜力有限。然而,在以干小麦麸为基础的日粮中添加木聚糖酶提高了NDF的AID以及GE和N的ATTD,但作为预处理的湿喂并没有进一步提高以小麦麸为基础日粮的营养价值。