Raza Waseem, Wei Zhong, Ling Ning, Huang Qiwei, Shen Qirong
Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural University, Wei Gang Road, No. 1, 210095, Nanjing, Jiangsu Province, PR China.
Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural University, Wei Gang Road, No. 1, 210095, Nanjing, Jiangsu Province, PR China.
J Biotechnol. 2016 Jun 10;227:43-53. doi: 10.1016/j.jbiotec.2016.04.014. Epub 2016 Apr 8.
Three organic fertilizers made of different animal and plant waste materials (BOFs) were evaluated for their effects on the production of antibacterial volatile organic compounds (VOCs) by two Bacillus amyloliquefaciens strains SQR-9 and T-5 against the tomato wilt pathogen Ralstonia solanacearum (RS). Both strains could produce VOCs that inhibited the growth and virulence traits of RS; however, in the presence of BOFs, the production of antibacterial VOCs was significantly increased. The maximum inhibition of growth and virulence traits of RS by VOCs of T-5 and SQR-9 was determined at 1.5% BOF2 and 2% BOF3, respectively. In case of strain T-5, 2-nonanone, nonanal, xylene, benzothiazole, and butylated hydroxy toluene and in case of strain SQR-9, 2-nonanone, nonanal, xylene and 2-undecanone were the main antibacterial VOCs whose production was increased in the presence of BOFs. The results of this study reveal another significance of using organic fertilizers to improve the antagonistic activity of biocontrol agents against phytopathogens.
评估了三种由不同动植物废料制成的有机肥料（生物有机肥）对两株解淀粉芽孢杆菌SQR-9和T-5产生抗番茄青枯病菌（RS）的抗菌挥发性有机化合物（VOCs）的影响。两株菌都能产生抑制RS生长和致病特性的VOCs；然而，在生物有机肥存在的情况下，抗菌VOCs的产量显著增加。T-5和SQR-9的VOCs对RS生长和致病特性的最大抑制作用分别在1.5%的生物有机肥2和2%的生物有机肥3时测定。对于T-5菌株，2-壬酮、壬醛、二甲苯、苯并噻唑和丁基化羟基甲苯，对于SQR-9菌株，2-壬酮、壬醛、二甲苯和2-十一酮是主要的抗菌VOCs，它们在生物有机肥存在时产量增加。本研究结果揭示了使用有机肥料提高生物防治剂对植物病原菌拮抗活性的另一个重要意义。
