State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
Guangdong Innovative Research Team of Sociomicrobiology, College of Agriculture, South China Agricultural University, Guangzhou 510642, China.
Molecules. 2018 Jan 19;23(1):214. doi: 10.3390/molecules23010214.
is a causative agent of bacterial wilt in many important crops throughout the world. How to control bacterial wilt caused by is a major problem in agriculture. In this study, we aim to isolate the biocontrol agents that have high efficacy in the control of bacterial wilt. Three new bacterial strains with high antimicrobial activity against GMI1000 were isolated and identified. Our results demonstrated that these bacteria could remarkably inhibit the disease index of host plant infected by . It was indicated that strain GZ-34 (CCTCC No. M 2016353) showed an excellent protective effect to tomato under greenhouse conditions. Strain GZ-34 was characterized as based on morphology, biochemistry, and 16S rRNA analysis. We identified that the main antimicrobial compounds produced by GZ-34 were cyclo(l-Pro-d-Ile) and cyclo(l-Pro-l-Phe) using electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) analysis. The two active compounds also interfered with the expression levels of some pathogenicity-contributors of . Furthermore, cyclo(l-Pro-l-Phe) effectively inhibited spore formation of , which is a vital pathogenesis process of the fungal pathogen, suggesting cyclic dipeptides from are promising potential antimicrobial agents with broad-spectrum activity to kill pathogens or interfere with their pathogenesis.
是一种在世界范围内许多重要作物中引起细菌性萎蔫病的病原体。如何控制由 引起的细菌性萎蔫病是农业中的一个主要问题。在这项研究中，我们旨在分离对细菌性萎蔫病具有高效控制作用的生防剂。从对 GMI1000 具有高抗菌活性的三种新细菌菌株中分离并鉴定出来。我们的结果表明，这些细菌可以显著抑制被 感染的宿主植物的疾病指数。结果表明，菌株 GZ-34（CCTCC No. M 2016353）在温室条件下对番茄表现出极好的保护作用。根据形态、生物化学和 16S rRNA 分析，菌株 GZ-34 被鉴定为 。我们通过电喷雾电离质谱（ESI-MS）和核磁共振（NMR）分析鉴定出，GZ-34 产生的主要抗菌化合物为环（l-Pro-d-Ile）和环（l-Pro-l-Phe）。这两种活性化合物还干扰了 的一些致病性因子的表达水平。此外，环（l-Pro-l-Phe）有效地抑制了 的孢子形成，这是真菌病原体的一个重要发病过程，这表明来自 的环二肽是具有广谱活性的有前途的潜在抗菌剂，可以杀死病原体或干扰其发病机制。
