Astiz M E, Rackow E C, Kim Y B, Weil M H
Department of Medicine, University of Health Sciences, Chicago Medical School, Illinois 60064.
Circ Shock. 1989 Mar;27(3):193-8.
The acute hemodynamic effects of Salmonella minnesota monophosphoryl lipid A (MPL) were compared with Salmonella minnesota lipopolysaccharide (LPS) in rats. Ten animals were randomized to receive either 0.25 mg MPL/100 g or 0.25 mg LPS/100 g intravenously. Arterial pressure (MAP), thermodilution cardiac output (CO), and colloid osmotic pressure (COP) were measured prior to and 30 and 60 min after MPL or LPS administration. In LPS-treated animals, CO decreased from 356 +/- 23 to 229 +/- 24 ml/kg/min at 30 min (P less than .01). MAP decreased from 115 +/- 7 to 89 +/- 2 mm Hg (P less than .05), and COP decreased from 18.4 +/- 0.6 to 15.4 +/- 0.8 mm Hg (P less than .05) at 60 min. In MPL-treated animals, no significant changes were observed in CO, MAP, and COP at 30 and 60 min. Intravenous infusion of MPL does not produce the hemodynamic derangements or increases in capillary permeability observed with endotoxin. These observations are consistent with previous reports demonstrating the limited toxicity of this lipid A derivative.
在大鼠中比较了明尼苏达沙门氏菌单磷酰脂质A（MPL）和明尼苏达沙门氏菌脂多糖（LPS）的急性血流动力学效应。将10只动物随机分为两组，分别静脉注射0.25mg MPL/100g或0.25mg LPS/100g。在注射MPL或LPS之前以及之后30分钟和60分钟测量动脉压（MAP）、热稀释心输出量（CO）和胶体渗透压（COP）。在LPS处理的动物中，30分钟时CO从356±23降至229±24ml/kg/min（P＜0.01）。60分钟时，MAP从115±7降至89±2mmHg（P＜0.05），COP从18.4±0.6降至15.4±0.8mmHg（P＜0.05）。在MPL处理的动物中，30分钟和60分钟时CO、MAP和COP未观察到显著变化。静脉输注MPL不会产生内毒素所导致的血流动力学紊乱或毛细血管通透性增加。这些观察结果与先前报道该脂质A衍生物毒性有限的研究一致。
