交感神经元上乙酰胆碱受体与α-银环蛇毒素结合成分之间的免疫学差异。
Immunological distinction between acetylcholine receptor and the alpha-bungarotoxin-binding component on sympathetic neurons.
作者信息
Patrick J, Stallcup W B
出版信息
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4689-92. doi: 10.1073/pnas.74.10.4689.
Abstract
Clone PC12 of a rat sympathetic neuron cell line binds alpha-bungarotoxin and exhibits carbamoylcholine-stimulated uptake of sodium ions. Concentrations of alpha-bungarotoxin that saturate the alpha-bungarotoxin-binding site have no effect on agonist-stimulated sodium uptake. Conversely, antibodies against eel acetylcholine receptor block the agonist-induced sodium flux but fail to recognize the alpha-bungarotoxin-binding component. Detergent extracts of the PC12 clone inhibit the ability of antibody to eel acetylcholine receptor to recognize 125I-alpha-bungarotoxin-acetylcholine receptor complexes derived from muscle. These results distinguish between a ganglionic nicotinic acetylcholine receptor and an alpha-bungarotoxin-binding component on these cells, and provide evidence for antigenic similarities between muscle acetylcholine receptor and ganglionic acetylcholine receptor.
摘要
大鼠交感神经元细胞系的克隆PC12能结合α-银环蛇毒素，并表现出氨甲酰胆碱刺激的钠离子摄取。使α-银环蛇毒素结合位点饱和的α-银环蛇毒素浓度对激动剂刺激的钠摄取没有影响。相反，抗鳗鱼乙酰胆碱受体的抗体可阻断激动剂诱导的钠通量，但无法识别α-银环蛇毒素结合成分。PC12克隆的去污剂提取物抑制抗鳗鱼乙酰胆碱受体抗体识别源自肌肉的125I-α-银环蛇毒素-乙酰胆碱受体复合物的能力。这些结果区分了这些细胞上的神经节烟碱型乙酰胆碱受体和α-银环蛇毒素结合成分，并为肌肉乙酰胆碱受体和神经节乙酰胆碱受体之间的抗原相似性提供了证据。
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Proc Natl Acad Sci U S A. 1977 Oct;74(10):4689-92. doi: 10.1073/pnas.74.10.4689.
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本文引用的文献
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Modes of actions of purified toxins from elapid venoms on neuromuscular transmission.眼镜蛇科蛇毒纯化毒素对神经肌肉传递的作用方式。
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Binding of (125I)-alpha bungarotoxin to particulate fractions of rat and guinea pig brain.（125I）-α-银环蛇毒素与大鼠和豚鼠脑微粒体部分的结合。
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Development of acetylcholine receptor clusters on cultured muscle cells.培养的肌肉细胞上乙酰胆碱受体簇的发育
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Acetylcholine receptors of muscle grown in vitro.体外培养的肌肉中的乙酰胆碱受体
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Appearance of acetylcholine receptors during differentiation of a myogenic cell line.乙酰胆碱受体在成肌细胞系分化过程中的出现。
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2762-6. doi: 10.1073/pnas.69.10.2762.
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Nicotinic cholinergic receptor in brain detected by binding of alpha-(3 H) bungarotoxin.通过α-（3H）银环蛇毒素结合检测脑中的烟碱型胆碱能受体。
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Effect of whole and fractionated cobra venom on sympathetic ganglionic transmission.全蝎毒和分级蝎毒对交感神经节传递的影响。 （注：原文说的是眼镜蛇毒，这里译文按“蝎毒”是错误的，正确译文应该是：眼镜蛇全毒及分级组分对交感神经节传递的影响。 ）
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Immunological characterisation of the cholinergic receptor protein from Electrophorus electricus.电鳗胆碱能受体蛋白的免疫学特性分析
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10
Clonal cell lines from the rat central nervous system.来自大鼠中枢神经系统的克隆细胞系。
Nature. 1974 May 17;249(454):224-7. doi: 10.1038/249224a0.
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