急性或慢性胆碱能刺激撤药后烟碱受体介导的钠-22内流的快速恢复。
Rapid recovery of nicotinic receptor-mediated sodium-22 influx following withdrawal from acute or chronic cholinergic stimulation.
作者信息
Siman R G, Klein W L
出版信息
Cell Mol Neurobiol. 1982 Sep;2(3):255-61. doi: 10.1007/BF00711153.
Abstract
- Acetylcholine receptor-mediated uptake of 22Na was studied in PC12 cells and 11-day chick muscle cells maintained in culture and exposed to carbamylcholine. 2. Carbamylcholine caused an initial 22-fold increase in the rate of 22Na uptake but this fell to less than twice background after 4-10 min of continuous exposure. The decline reflects receptor desensitization. 3. The effects of acute (10-min) and chronic (10-day) exposure were compared in order to determine whether there was a down-regulation of acetylcholine receptors on chronic exposure to carbamylcholine. No down-regulation was observed on either PC12 or muscle cells. 4. The lack of down-regulation in these nicotinic systems contrasts with results on muscarinic systems and may reflect different roles for these receptor types.
摘要
- 在培养的PC12细胞和11日龄鸡肌肉细胞中研究了乙酰胆碱受体介导的22Na摄取，并使其暴露于氨甲酰胆碱。2. 氨甲酰胆碱使22Na摄取速率最初增加了22倍，但在连续暴露4 - 10分钟后降至低于背景值的两倍。这种下降反映了受体脱敏。3. 比较了急性（10分钟）和慢性（10天）暴露的影响，以确定长期暴露于氨甲酰胆碱时乙酰胆碱受体是否下调。在PC12细胞或肌肉细胞中均未观察到下调。4. 这些烟碱样系统中缺乏下调与毒蕈碱样系统的结果形成对比，这可能反映了这些受体类型的不同作用。
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