通过DNA含量和C带对Y染色体多态性进行分类。

Classification of Y chromosome polymorphisms by DNA content and C-banding.

作者信息

Wall W J, Butler L J

机构信息

Department of Cytogenetics, Queen Elizabeth Hospital For Children, London, UK.

出版信息

Chromosoma. 1989 Jan;97(4):296-300. doi: 10.1007/BF00371969.

DOI:10.1007/BF00371969

PMID:2707100

Abstract

A system has been developed using a microscope photometer to measure the DNA content of individual chromosomes. After correcting for the systematic and statistical errors the measured values are highly reproducible. Data are presented of measurements of human Y chromosomes from a cytogenetically normal population. When compared with the C-banding pattern from the same specimens the DNA content is found to change in discrete steps of 1.36 x 10(-14)g or 13.26 x 10(6) bp for each of the Y chromosome polymorphic types.

摘要

已开发出一种使用显微镜光度计测量单个染色体DNA含量的系统。在校正系统误差和统计误差后，测量值具有高度可重复性。给出了来自细胞遗传学正常人群的人类Y染色体测量数据。与相同标本的C带模式相比，发现每种Y染色体多态性类型的DNA含量以1.36×10⁻¹⁴克或13.26×10⁶碱基对的离散步长变化。