Newbold R R, Pentecost B T, Yamashita S, Lum K, Miller J V, Nelson P, Blair J, Kong H, Teng C, McLachlan J A
Developmental Endocrinology and Pharmacology Section, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Endocrinology. 1989 May;124(5):2568-76. doi: 10.1210/endo-124-5-2568.
Previous studies from our laboratory on the feminization of the male mouse reproductive tract after prenatal exposure to diethylstilbestrol (DES) showed that the mRNA for the major estrogen-inducible uterine secretory protein, lactoferrin (LF), was constitutively expressed in the seminal vesicle of male mice exposed prenatally to DES, but not in the seminal vesicle of control mice. After castration, treatment with 17 beta-estradiol (20 micrograms/kg.day) for 3 days induced the LF mRNA in the seminal vesicle of both control and prenatally DES-exposed mice; however, the levels in DES-treated tissues were approximately 6-fold higher than those in control tissue. This report describes the presence of LF in seminal vesicle tissues and secretions of prenatally DES-exposed mice, as determined by immunohistochemistry and Western blot analysis. Further, these data are correlated with immunolocalization of the estrogen receptor in the seminal vesicle tissue. We conclude that the seminal vesicle of prenatally DES-exposed male mice has acquired two key characteristics of female tissues, namely LF production/regulation and estrogen receptor localization/distribution similar to that in uterine tissues.
我们实验室之前关于产前暴露于己烯雌酚（DES）后雄性小鼠生殖道雌性化的研究表明，主要的雌激素诱导型子宫分泌蛋白乳铁蛋白（LF）的mRNA在产前暴露于DES的雄性小鼠精囊中组成性表达，但在对照小鼠的精囊中不表达。去势后，用17β-雌二醇（20微克/千克·天）处理3天可诱导对照小鼠和产前暴露于DES的小鼠精囊中LF mRNA的表达；然而，DES处理组织中的水平比对照组织中的水平高约6倍。本报告描述了通过免疫组织化学和蛋白质印迹分析确定的产前暴露于DES的小鼠精囊组织和分泌物中LF的存在情况。此外，这些数据与雌激素受体在精囊组织中的免疫定位相关。我们得出结论，产前暴露于DES的雄性小鼠的精囊获得了雌性组织的两个关键特征，即LF的产生/调节以及雌激素受体的定位/分布，类似于子宫组织中的情况。
