JebaMercy Gnanasekaran, Durai Sellegounder, Prithika Udayakumar, Marudhupandiyan Shanmugam, Dasauni Pushpanjali, Kundu Suman, Balamurugan Krishnaswamy
Department of Biotechnology, Alagappa University, Karaikudi 630004, India.
Department of Biochemistry, University of Delhi South Campus, New Delhi 110021, India.
J Proteomics. 2016 Aug 11;145:81-90. doi: 10.1016/j.jprot.2016.03.047. Epub 2016 Apr 9.
Caenorhabditis elegans is emerging as one of the handy model for proteome related studies due to its simplest system biology. The present study, deals with changes in protein expression in C. elegans infected with Proteus mirabilis. Proteins were separated using two-dimensional differential gel electrophoresis (2D-DIGE) and identified using MALDI-TOF. Twelve distinctly regulated proteins identified in the infected worms, included heat shock proteins involved stress pathway (HSP-1 and HSP-6), proteins involved in immune response pathway (DAF-21), enzymes involved in normal cellular process (Eukaryotic translation Elongation Factor, actin family member, S-adenosyl homocysteine hydrolase ortholog, glutamate dehydrogenase and Vacuolar H ATPase family member) and few least characterized proteins (H28O16.1 and H08J11.2). The regulation of selected players at the transcriptional level during Proteus mirabilis infection was analyzed using qPCR. Physiological experiments revealed the ability of P. mirabilis to kill daf-21 mutant C. elegans significantly compared with the wild type. This is the first report studying proteome changes in C. elegans and exploring the involvement of MAP Kinase pathway during P. mirabilis infection.
This is the first report studying proteome changes in C. elegans during P. mirabilis infection. The present study explores the role and contribution of MAP Kinase pathway and its regulator protein DAF-21 involvement in the immunity against opportunistic pathogen P. mirabilis infection. Manipulation of this DAF-21 protein in host, may pave the way for new drug development or disease control strategy during opportunistic pathogen infections.
由于其最简单的系统生物学特性，秀丽隐杆线虫正成为蛋白质组相关研究的便捷模型之一。本研究探讨了奇异变形杆菌感染秀丽隐杆线虫后蛋白质表达的变化。使用二维差异凝胶电泳（2D-DIGE）分离蛋白质，并使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）进行鉴定。在受感染的线虫中鉴定出12种明显受调控的蛋白质，包括参与应激途径的热休克蛋白（HSP-1和HSP-6）、参与免疫反应途径的蛋白质（DAF-21）、参与正常细胞过程的酶（真核翻译延伸因子、肌动蛋白家族成员、S-腺苷同型半胱氨酸水解酶直系同源物、谷氨酸脱氢酶和液泡H-ATP酶家族成员）以及一些特征最少的蛋白质（H28O16.1和H08J11.2）。使用实时定量聚合酶链反应（qPCR）分析了奇异变形杆菌感染期间选定蛋白在转录水平的调控。生理学实验表明，与野生型相比，奇异变形杆菌显著杀死daf-21突变型秀丽隐杆线虫的能力。这是第一项研究秀丽隐杆线虫蛋白质组变化并探索丝裂原活化蛋白激酶（MAP）激酶途径在奇异变形杆菌感染过程中作用的报告。
这是第一项研究奇异变形杆菌感染期间秀丽隐杆线虫蛋白质组变化的报告。本研究探讨了MAP激酶途径及其调节蛋白DAF-21在抵抗机会性病原体奇异变形杆菌感染的免疫中的作用和贡献。在宿主中操纵这种DAF-21蛋白，可能为机会性病原体感染期间的新药开发或疾病控制策略铺平道路。
