用于评估不透明样品的垂直缓解密度测定法。
Vertical remission densitometry for the evaluation of nontransparent samples.
作者信息
Taube D, Neuhoff V
机构信息
Max-Planck-Institut für experimentelle Medizin, Forschungsstelle Neurochemie, Göttingen, Federal Republic of Germany.
出版信息
Electrophoresis. 1989 Mar;10(3):215-7. doi: 10.1002/elps.1150100310.
Gels dried onto filter paper or their blots on membranes are not suitable for densitometric evaluations in the transmission mode because these samples are nearly nontransparent. By scanning in the remission mode, evaluation with good results is possible. Data acquisition by a computer-controlled high speed scanning photometer and digital image processing affords a spatial resolution and accuracy similar to that in the transmission mode. The signal-to-background ratio decreases by 10-30% depending on the smoothness of the support.
干燥在滤纸上的凝胶或其在膜上的印迹不适合在透射模式下进行光密度评估，因为这些样品几乎不透明。通过在反射模式下扫描，可以进行效果良好的评估。通过计算机控制的高速扫描光度计进行数据采集和数字图像处理，可提供与透射模式相似的空间分辨率和精度。根据支持物的光滑程度，信噪比会降低10%-30%。
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