用于更高灵敏度和分辨率的镜像密度测定法。
Mirror densitometry for higher sensitivity and resolution.
作者信息
Taube D, Neuhoff V
机构信息
Max-Planck-Institut für experimentelle Medizin, Forschungstelle Neurochemie, Göttingen, Federal Republic of Germany.
出版信息
Electrophoresis. 1989 Mar;10(3):218-9. doi: 10.1002/elps.1150100311.
PMID:2707245
Abstract
In order to increase the pathway of the light inside a gel (or autoradiogram) during scanning, it is placed on top of a mirror and the densitometry is performed in a vertical reflection mode. As the light passes the gel a second time after being reflected by the mirror, the absorbance is nearly doubled. This increase in absorbance results in higher sensitivity and resolution.
摘要
为了在扫描过程中增加光线在凝胶（或放射自显影片）内的传播路径，将其放置在镜子上方，并以垂直反射模式进行光密度测定。由于光线在被镜子反射后第二次穿过凝胶，吸光度几乎增加了一倍。吸光度的这种增加导致更高的灵敏度和分辨率。
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