Ohsawa T
Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.
Exp Gerontol. 1989;24(1):1-9. doi: 10.1016/0531-5565(89)90030-2.
The gangliosides in human diploid fibroblasts--TIG-1, TIG-7, and IMR-90--were analysed at different cell densities at early and late passages to clarify the relationship between age and cell density dependent changes of the gangliosides. In early passages, the ganglioside concentrations increased with increase in cell density. At late passages, however, the concentrations were lower than those at the early passages either in the growing or confluent phase, and slightly increased with increase in cell density. The pattern of ganglioside compositions were apparently different between early and late passage cells either in growing or confluent state. In the early passages, GM3 and GD3 were major constituents, and GM2, GD1a, or the other more complex gangliosides were detected as minor components. With increase in cell density, the content of GM3 decreased, whereas GD3 and the others increased. At the late passages, however, GM3 was the major component, and GD3, GM2, or GD1a were minor, but the others were hardly detectable. The ganglioside pattern did not change with increase in cell density. Thus, the age-dependent changes of gangliosides could be distinguished from the cell density dependent alterations.
对人二倍体成纤维细胞TIG - 1、TIG - 7和IMR - 90在不同传代早期和晚期的不同细胞密度下的神经节苷脂进行分析，以阐明年龄与神经节苷脂细胞密度依赖性变化之间的关系。在传代早期，神经节苷脂浓度随细胞密度增加而升高。然而，在传代后期，无论是在生长阶段还是汇合阶段，其浓度均低于传代早期，且随细胞密度增加略有升高。无论是在生长状态还是汇合状态下，传代早期和晚期细胞的神经节苷脂组成模式明显不同。在传代早期，GM3和GD3是主要成分，GM2、GD1a或其他更复杂的神经节苷脂作为次要成分被检测到。随着细胞密度增加，GM3含量降低，而GD3和其他成分增加。然而，在传代后期，GM3是主要成分，GD3、GM2或GD1a是次要成分，但其他成分几乎检测不到。神经节苷脂模式不随细胞密度增加而变化。因此，神经节苷脂的年龄依赖性变化可以与细胞密度依赖性改变区分开来。
