Chaabane Chaouch Fawzia, Bouras Noureddine, Mokrane Salim, Zitouni Abdelghani, Schumann Peter, Spröer Cathrin, Sabaou Nasserdine, Klenk Hans-Peter
Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, BP 455, Ghardaïa 47000, Algeria.
Int J Syst Evol Microbiol. 2016 Jul;66(7):2484-2490. doi: 10.1099/ijsem.0.001077. Epub 2016 Apr 13.
The taxonomic position of a novel actinobacterium, strain SG1, isolated from a Saharan soil sample collected from Béni-Abbès, Béchar (south-west Algeria), was established by using a polyphasic approach. The micro-organism had morphological and chemical features that were consistent with its classification in the genus Streptosporangium. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose and glucose, but not madurose. The predominant menaquinones were MK-9(H2) and MK-9(H4). The polar lipid profile contained diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylhydroxymethylethanolamine, phosphatidylhydroxyethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The predominant cellular fatty acids were C17 : 1ω8c, iso-C16 : 0, 10-methyl C17 : 0, C18 : 1ω9c and C17 : 0. 16S rRNA gene sequence similarity analysis supported the classification of the isolate in the genus Streptosporangium and indicated that it was related most closely to 'Streptosporangium subfuscum' DSM 46724 (99.7 % similarity), Streptosporangium pseudovulgare DSM 43181 (98.7 %), Streptosporangium fragile DSM 43847 (98.6 %) and Streptosporangium sandarakinum DSM 45763 (98.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SG1 formed a cluster with its closest relative 'S. subfuscum' DSM 46724. However, DNA-DNA relatedness as well as physiological and chemotaxonomical analyses showed that strain SG1 could be differentiated from its closest phylogenetic relatives. Therefore, it is proposed that strain SG1 should be classified as representing a novel species in the genus Streptosporangium, for which the name Streptosporangiumbecharense sp. nov. is proposed. The type strain is SG1 (=DSM 46887=CECT 8961).
采用多相分类法确定了从阿尔及利亚西南部贝沙尔省贝尼-阿贝斯采集的撒哈拉土壤样品中分离出的一株新型放线菌SG1的分类地位。该微生物具有与链孢囊菌属分类一致的形态和化学特征。细胞壁肽聚糖含有内消旋二氨基庚二酸。全细胞糖含有核糖和葡萄糖，但不含马杜拉糖。主要的甲基萘醌是MK-9(H2)和MK-9(H4)。极性脂质谱含有双磷脂酰甘油、磷脂酰甲基乙醇胺、磷脂酰乙醇胺、磷脂酰羟甲基乙醇胺、磷脂酰羟乙醇胺、磷脂酰甘油、磷脂酰肌醇和磷脂酰肌醇甘露糖苷。主要的细胞脂肪酸是C17 : 1ω8c、异-C16 : 0、10-甲基-C17 : 0、C18 : 1ω9c和C17 : 0。16S rRNA基因序列相似性分析支持该分离株属于链孢囊菌属，并表明它与“暗褐链孢囊菌”DSM 46724（相似性99.7%）、假普通链孢囊菌DSM 43181（98.7%）、脆弱链孢囊菌DSM 43847（98.6%）和沙黄链孢囊菌DSM 45763（98.5%）关系最为密切。基于16S rRNA基因序列的系统发育分析表明，菌株SG1与其最接近的亲缘种“暗褐链孢囊菌”DSM 46724形成一个簇。然而，DNA-DNA相关性以及生理和化学分类分析表明，菌株SG1可以与其最接近的系统发育亲缘种区分开来。因此，建议将菌株SG1分类为链孢囊菌属中的一个新物种，命名为贝沙尔链孢囊菌（Streptosporangium becharense）新种。模式菌株为SG1（=DSM 46887=CECT 8961）。
