Suriyachadkun Chanwit, Ngaemthao Wipaporn, Chunhametha Suwanee, Thawai Chitti, Sanglier Jean-Jacques
BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand.
Department of Biology and Actinobacterial Research Unit, Faculty of Science, King Monkut's Institute of Technology Ladkrabang, Bangkok, Thailand.
Int J Syst Evol Microbiol. 2015 Jun;65(Pt 6):1764-1769. doi: 10.1099/ijs.0.000172. Epub 2015 Mar 5.
A novel actinomycete, A-T 8343T was isolated from a moist evergreen forest soil sample collected in the Trat Province, Thailand. Based on 16S rRNA gene sequence analysis, strain A-T 8343T belonged to the genus Sinosporangium and was closely related to Sinosporangium siamense A-T 1946T (98.81 %) and Sinosporangium album 6014T (98.54 %). The DNA-DNA relatedness values were 21.8-27 % with S. siamense A-T 1946T and 31.1-31.9 % with S. album 6014T, which were significantly below 70 %. The result differentiated A-T 8343T from the closest species. The organism developed spherical sporangia containing non-motile spores on aerial mycelia. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained rhamnose, ribose, madurose and glucose. The predominant menaquinones were MK-9(H2) and MK-9(H4). The diagnostic phospholipids were phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyl-phosphatidylethanolamine, diphosphatidylglycerol, lyso-phosphatidylethanolamine, N-acetylglucosamine-containing phospholipids, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol-mannosides, aminophosphoglycolipid and one unknown phospholipid. The major cellular fatty acids were saturated C16 : 0, iso C16 : 0, unsaturated C16 : 1 and C18 : 1. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the new isolate is proposed as a representative novel species of the genus Sinosporangium to be named Sinosporangiumfuscum sp. nov. The type strain is A-T 8343T ( = BCC 52770T = NBRC 109516T).
从泰国桐艾府采集的湿润常绿森林土壤样本中分离出一株新型放线菌A-T 8343T。基于16S rRNA基因序列分析，菌株A-T 8343T属于中华孢囊菌属，与暹罗中华孢囊菌A-T 1946T（98.81%）和白色中华孢囊菌6014T（98.54%）密切相关。其与暹罗中华孢囊菌A-T 1946T的DNA-DNA杂交值为21.8-27%，与白色中华孢囊菌6014T的DNA-DNA杂交值为31.1-31.9%，均显著低于70%。该结果将A-T 8343T与最接近的物种区分开来。该菌在气生菌丝体上形成含有不运动孢子的球形孢囊。细胞壁肽聚糖含有内消旋二氨基庚二酸。全细胞糖含有鼠李糖、核糖、马杜拉糖和葡萄糖。主要的甲基萘醌是MK-9(H2)和MK-9(H4)。诊断性磷脂为磷脂酰甲基乙醇胺、磷脂酰乙醇胺、羟基磷脂酰乙醇胺、二磷脂酰甘油、溶血磷脂酰乙醇胺、含N-乙酰葡糖胺的磷脂、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、氨基磷酸糖脂和一种未知磷脂。主要的细胞脂肪酸为饱和C16 : 0、异C16 : 0、不饱和C16 : 1和C18 : 1。在对表型、化学分类和基因型特征进行评估后，提议将该新分离株作为中华孢囊菌属的一个代表性新物种，命名为暗褐中华孢囊菌（Sinosporangiumfuscum sp. nov.）。模式菌株为A-T 8343T（= BCC 52770T = NBRC 109516T）。
