Pittenger Steven T, Swalve Natashia, Chou Shinnyi, Smith Misty D, Hoonakker Amanda J, Pudiak Cindy M, Fleckenstein Annette E, Hanson Glen R, Bevins Rick A
Department of Psychology, University of Nebraska-Lincoln, 238 Burnett Hall, Lincoln, Nebraska, 68588-0308.
School of Dentistry, University of Utah, 530 so. Wakara Way, Salt Lake City, Utah, 84108.
Synapse. 2016 Aug;70(8):336-46. doi: 10.1002/syn.21907. Epub 2016 May 4.
Investigator-administered nicotine alters neurotensin and substance P levels in Sprague-Dawley rats. This finding suggested a role of the dopamine-related endogenous neuropeptides in nicotine addiction. We sought to extend this observation by determining the responses of neurotensin and substance P systems (assessed using radioimmunoassay) in male and female rats following nicotine self-administration (SA). Male and female Sprague-Dawley were trained to self-administer nicotine, or receive saline infusions yoked to a nicotine-administering rat during daily sessions (1-h; 21 days). Brains were extracted 3 h after the last SA session. Nicotine SA increased tissue levels of neurotensin in the males in the anterior and posterior caudate, globus pallidus, frontal cortex, nucleus accumbens core and shell, and ventral tegmental area. Nicotine SA also increased tissue levels of neurotensin in the females in the anterior caudate, globus pallidus, nucleus accumbens core and shell, but not in the posterior caudate, frontal cortex, or ventral tegmental area. There were fewer sex differences observed in the substance P systems. Nicotine SA increased tissue levels of substance P in both the males and females in the posterior caudate, globus pallidus, frontal cortex, nucleus accumbens shell, and ventral tegmental area. A sex difference was observed in the nucleus accumbens core, where nicotine SA increased tissue levels of substance P in the males, yet decreased levels in the females. The regulation of neuropeptides following nicotine SA may play a role in the susceptibility to nicotine dependence in females and males. Synapse 70:336-346, 2016. © 2016 Wiley Periodicals, Inc.
研究者给予的尼古丁会改变斯普拉格-道利大鼠体内神经降压素和P物质的水平。这一发现提示了多巴胺相关内源性神经肽在尼古丁成瘾中的作用。我们试图通过测定尼古丁自我给药（SA）后雄性和雌性大鼠神经降压素和P物质系统的反应（采用放射免疫分析法评估）来扩展这一观察结果。雄性和雌性斯普拉格-道利大鼠接受训练以自我给药尼古丁，或在每日时段（1小时；21天）接受与尼古丁给药大鼠相连的生理盐水输注。在最后一次SA实验结束3小时后取出大脑。尼古丁自我给药增加了雄性大鼠前、后尾状核、苍白球、额叶皮质、伏隔核核心和壳以及腹侧被盖区的神经降压素组织水平。尼古丁自我给药也增加了雌性大鼠前尾状核、苍白球、伏隔核核心和壳的神经降压素组织水平，但在后尾状核、额叶皮质或腹侧被盖区未增加。在P物质系统中观察到的性别差异较少。尼古丁自我给药增加了雄性和雌性大鼠后尾状核、苍白球、额叶皮质、伏隔核壳和腹侧被盖区的P物质组织水平。在伏隔核核心观察到性别差异，尼古丁自我给药增加了雄性大鼠的P物质组织水平，而降低了雌性大鼠的水平。尼古丁自我给药后神经肽的调节可能在雌性和雄性对尼古丁依赖的易感性中起作用。《突触》70:3&36 - 346，2016年。© 2016威利期刊公司。
