Lee Young Ji, Han Soo Jung, Lee Hun, Kim Jin Sun, Seo Kyoung Yul
Yonsei Plus Eye Clinic Seongnam, South Korea 2Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, South Korea.
Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, South Korea.
Invest Ophthalmol Vis Sci. 2016 Apr;57(4):1773-81. doi: 10.1167/iovs.15-17340.
The purpose of this study was to develop a murine model of allergic conjunctivitis induced by house dust mite (HDM) extract from Dermatophagoides pteronyssinus, a major allergen in humans.
Forty BALB/c mice were divided into five groups, immunized with placebo, ovalbumin (10 μg), or HDM extract following a schedule. Twenty minutes after topical challenge, mice were examined clinically. Material collected from mice was used for measuring total and specific IgE, antigen-specific lymphocyte proliferation, and supernatant cytokine levels and for conjunctival histopathology and flow cytometric analysis of conjunctival cells.
This murine model showed similar clinical signs and laboratory findings to human allergy and the ovalbumin-induced allergic conjunctivitis model. Total IgE levels and conjunctival infiltration of mast cells and eosinophils in immunized mice were significantly higher than in the control group. Cervical lymphocyte proliferation was increased in antigen-stimulated cultures in immunized mice, concomitant with significantly higher IL-4 and IL-5 levels in the culture supernatant. The proportion of conjunctival CD4+ T cells expressing the ST2 receptor was increased, and conjunctival CD4+ST2+ T cells exhibited an increase in intracellular IL-5.
House dust mite extract successfully induced allergic conjunctivitis in BALB/c mice. Ten micrograms of HDM extract was the optimal dose for systemic immunization in this model. This murine model is suitable for further studies on HDM-induced allergic conjunctivitis, and the data show that conjunctival CD4+ T cells expressing ST2 may play an important role in IL-5 secretion, recruiting eosinophils into conjunctiva on ocular allergen challenge.
本研究旨在建立一种由屋尘螨（HDM）提取物诱导的变应性结膜炎小鼠模型，该提取物来自人类主要变应原柏氏禽刺螨。
将40只BALB/c小鼠分为五组，按照一定的免疫程序分别用安慰剂、卵清蛋白（10μg）或屋尘螨提取物进行免疫。局部激发后20分钟，对小鼠进行临床检查。从小鼠收集的材料用于测量总IgE和特异性IgE、抗原特异性淋巴细胞增殖、上清细胞因子水平，并用于结膜组织病理学检查和结膜细胞的流式细胞术分析。
该小鼠模型表现出与人类变应性疾病及卵清蛋白诱导的变应性结膜炎模型相似的临床症状和实验室检查结果。免疫小鼠的总IgE水平以及肥大细胞和嗜酸性粒细胞在结膜的浸润均显著高于对照组。免疫小鼠抗原刺激培养物中的颈部淋巴细胞增殖增加，同时培养上清液中的IL-4和IL-5水平显著升高。表达ST2受体的结膜CD4+T细胞比例增加，结膜CD4+ST2+T细胞的细胞内IL-5增加。
屋尘螨提取物成功诱导BALB/c小鼠发生变应性结膜炎。10μg的屋尘螨提取物是该模型全身免疫的最佳剂量。该小鼠模型适用于进一步研究屋尘螨诱导的变应性结膜炎，数据表明表达ST2的结膜CD4+T细胞可能在IL-5分泌中起重要作用，在眼部变应原激发时将嗜酸性粒细胞募集到结膜中。
