富含多酚的复合植物提取物对高脂饮食诱导的 C57BL/6 小鼠肥胖相关代谢功能紊乱及非酒精性脂肪性肝病的保护作用。

Protective Effects of Polyphenol Enriched Complex Plants Extract on Metabolic Dysfunctions Associated with Obesity and Related Nonalcoholic Fatty Liver Diseases in High Fat Diet-Induced C57BL/6 Mice.

机构信息

Lee's Biotech Co., Ltd. #416, C Dong, 17 Techno 4-ro, Yuseon-gu, Daejeon 34013, Korea.

Myunggok Medical Research Institute, College of Medicine, Konyang University, Daejeon 35365, Korea.

出版信息

Molecules. 2021 Jan 8;26(2):302. doi: 10.3390/molecules26020302.

BACKGROUND

Currently, obesity is a global health challenge due to its increasing prevalence and associated health risk. It is associated with various metabolic diseases, including diabetes, hypertension, cardiovascular disease, stroke, certain forms of cancer, and non-alcoholic liver diseases (NAFLD).

OBJECTIVE

The aim of this study to evaluate the effects of polyphenol enriched herbal complex (/ellagic acid, Bunge/vitexin, chlorogenic acid, /cinnamic acid) on obesity and obesity induced NAFLD in the high-fat diet (HFD)-induced obese mouse model.

METHODS

Obesity was induced in male C57BL/6 mice using HFD. After 8 weeks, the mice were treated with HFD+ plants extract for 8 weeks. Body weight, food intake weekly, and blood sugar level were measured. After sacrifice, changes in the treated group's liver weight, fat weight, serum biochemical parameters, hormone levels, and enzyme levels were measured. For histological analysis, tissues were stained with hematoxylin-eosin (H&E) and Oil Red-O.

RESULTS

Our results showed that the herbal complex ameliorated body weight and liver weight gain, and decreased total body fat in HFD-fed animals. Post prandial blood glucose (PBG) and fasting blood glucose (FBG) were lower in the herbal complex-treated group than in the HFD control group. Additionally, herbal formulation treatment significantly increased HDL levels in serum and decreased TC, TG, AST, ALT, deposition of fat droplets in the liver, and intima media thickness (IMT) in the aorta. Herbal complex increased serum adiponectin and decreased serum leptin. Herbal complex also increased carnitine palmityl transferase (CPT) activity and significantly decreased enzyme activity of beta-hydroxy beta methyl glutamyl-CoA (HMG-CoA) reductase, and fatty acid synthase (FAS).

CONCLUSIONS

The results of this study demonstrated that the herbal complex is an effective herbal formulation in the attenuation of obesity and obesity-induced metabolic dysfunction including NAFLD in HFD-induced mouse model.

背景

目前，肥胖是一个全球性的健康挑战，因为它的患病率不断增加，并且与健康风险有关。它与各种代谢疾病有关，包括糖尿病、高血压、心血管疾病、中风、某些形式的癌症和非酒精性肝病（NAFLD）。

目的

本研究旨在评估富含多酚的草药复合物（/鞣花酸、/Bunge/牡荆素、绿原酸、/肉桂酸）对高脂肪饮食（HFD）诱导肥胖小鼠模型中肥胖和肥胖诱导的非酒精性脂肪性肝病（NAFLD）的影响。

方法

使用 HFD 诱导雄性 C57BL/6 小鼠肥胖。8 周后，用 HFD+植物提取物处理 8 周。测量体重、每周食物摄入量和血糖水平。处死动物后，测量处理组的肝重、脂肪重、血清生化参数、激素水平和酶水平的变化。为了进行组织学分析，用苏木精-伊红（H&E）和油红-O 对组织进行染色。

结果

我们的结果表明，该草药复合物改善了 HFD 喂养动物的体重和肝重增加，并减少了总体脂肪。餐后血糖（PBG）和空腹血糖（FBG）在草药复合物治疗组低于 HFD 对照组。此外，草药配方治疗显著增加了血清中的高密度脂蛋白（HDL）水平，并降低了 TC、TG、AST、ALT、肝脏脂肪滴沉积和主动脉内膜中层厚度（IMT）。草药复合物增加了血清脂联素水平，降低了血清瘦素水平。草药复合物还增加了肉碱棕榈酰转移酶（CPT）活性，显著降低了羟甲基戊二酰基辅酶 A（HMG-CoA）还原酶和脂肪酸合酶（FAS）的酶活性。