Dizeux Alexandre, Payen Thomas, Barrois Guillaume, Le Guillou Buffello Delphine, Bridal S Lori
Sorbonne Universités, UPMC Univ Paris 06, CNRS, INSERM, Laboratoire d'Imagerie Biomédicale, F-75006, Paris, France.
Mol Imaging Biol. 2016 Oct;18(5):651-8. doi: 10.1007/s11307-016-0952-y.
Sensitivity of contrast-enhanced ultrasound (CEUS) to microvascular flow modifications can be limited by intra-injection variability (injected dose, rate, volume).
To evaluate the effect of injection variability on microvascular flow evaluation, CEUS was compared between controlled and manual injections where enhancement was assessed in vitro within a flow phantom, in normal murine kidney (N = 12) and in murine ectopic tumors (N = 10).
For both in vitro and in vivo measurements in the renal cortex, controlled injections significantly improved reproducibility of functional parameter estimation. Their coefficient of variation (CV) in the renal cortex ranged from 4 to 19 % for controlled injection vs. 5 to 43 % for manual injections. For measurements in tumors, controlled injection only decreased the CV significantly for the mean transit time. In tumors, multiple injections of contrast agent with a 15-min delay between each were shown to strongly modify contrast uptake by facilitating penetration of microbubbles.
Improved reproducibility of CEUS assessments in murine models should provide more robust quantification of flow parameters and more sensitive evaluation of tumor modifications in therapeutic models.
超声造影（CEUS）对微血管血流改变的敏感性可能受注射过程中的变异性（注射剂量、速率、体积）限制。
为评估注射变异性对微血管血流评估的影响，在体外血流模型、正常小鼠肾脏（n = 12）和小鼠异位肿瘤（n = 10）中，对控制注射和手动注射的CEUS进行比较，在体外对血流模型中的增强情况进行评估。
对于肾皮质的体外和体内测量，控制注射显著提高了功能参数估计的可重复性。在肾皮质中，控制注射的变异系数（CV）为4%至19%，而手动注射为5%至43%。对于肿瘤测量，控制注射仅显著降低了平均通过时间的CV。在肿瘤中，每次间隔15分钟多次注射造影剂显示，通过促进微泡渗透，强烈改变了造影剂摄取。
在小鼠模型中提高CEUS评估的可重复性，应能在治疗模型中更可靠地定量血流参数，并更敏感地评估肿瘤改变。
