Krieg P A, Varnum S M, Wormington W M, Melton D A
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Dev Biol. 1989 May;133(1):93-100. doi: 10.1016/0012-1606(89)90300-x.
A Xenopus laevis gastrula cDNA library has been screened in order to identify sequences that are expressed early in development. We find that the mRNA encoding translation elongation factor 1-alpha (EF-1 alpha) is synthesized in very large amounts in the early embryo. Transcription of EF-1 alpha mRNA commences at the midblastula transition (MBT), and new EF-1 alpha protein is synthesized very soon after this, as determined by the association of EF-1 alpha mRNA with polysomes. The nucleotide sequence of a full-length EF-1 alpha cDNA clone and the deduced amino acid sequence of Xenopus EF-1 alpha protein are presented.
为了鉴定在发育早期表达的序列，对非洲爪蟾原肠胚cDNA文库进行了筛选。我们发现，编码翻译延伸因子1-α（EF-1α）的mRNA在早期胚胎中大量合成。EF-1α mRNA的转录在囊胚中期转换（MBT）开始，并且在此之后很快就合成了新的EF-1α蛋白，这是通过EF-1α mRNA与多核糖体的结合来确定的。本文给出了全长EF-1α cDNA克隆的核苷酸序列以及非洲爪蟾EF-1α蛋白的推导氨基酸序列。
