Zhu Zhen-Yuan, Liu Xiao-Cui, Tang Ya-Li, Dong Feng-Ying, Sun Hui-Qing, Chen Lu, Zhang Yong-Min
Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China; Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, Tianjin 300457, PR China.
Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
J Biosci Bioeng. 2016 Oct;122(4):494-8. doi: 10.1016/j.jbiosc.2016.03.015. Epub 2016 Apr 10.
The effects of culture medium composition (i.e., carbon and nitrogen sources) on the growth of mycelia, molecular weight distribution and antitumor activity of intracellular polysaccharides (IPS) from Cordyceps gunnii were investigated. Sucrose and peptone were proved to be the best carbon and nitrogen sources for mycelia growth and remarkably improved IPS production. When the sucrose concentration was 2.0%, the mycelium yield reached up to 15.94±1.26 g/L, but with lower IPS yield; whereas the sucrose concentration was 4.5%, IPS yield reached to a maximum of 138.78±3.89 mg/100 mL. The effects of different carbon/nitrogen (C/N) ratios with equal amounts of carbon source matter on the mycelia and IPS formation were optimized. It found that the yield of mycelia and IPS were both reached to the highest at a C/N ratio of 10:3. In addition, the IPS had the highest macro molecular polysaccharide content and antitumor activity when sucrose concentration was 3.5% and the C/N ratio was 10:1.5. Thus, there was a positive correlation between molecular weight distribution and antitumor activity of IPS by C. gunnii.
研究了培养基组成（即碳源和氮源）对古尼虫草菌丝体生长、胞内多糖（IPS）分子量分布及抗肿瘤活性的影响。结果表明，蔗糖和蛋白胨是菌丝体生长的最佳碳源和氮源，显著提高了IPS的产量。当蔗糖浓度为2.0%时，菌丝体产量可达15.94±1.26 g/L，但IPS产量较低；而蔗糖浓度为4.5%时，IPS产量最高可达138.78±3.89 mg/100 mL。优化了等量碳源物质不同碳氮比（C/N）对菌丝体和IPS形成的影响。结果发现，当C/N比为10:3时，菌丝体和IPS的产量均达到最高。此外，当蔗糖浓度为3.5%且C/N比为10:1.5时，IPS的大分子多糖含量和抗肿瘤活性最高。因此，古尼虫草IPS的分子量分布与抗肿瘤活性之间存在正相关。
