Wang Lan-Ying, Cheong Kit-Leong, Wu Ding-Tao, Meng Lan-Zhen, Zhao Jing, Li Shao-Ping
State Key Laboratory of Quality Research in Chinese Medicine, and Institute of Chinese Medical Sciences, University of Macau, Macau SAR, China.
State Key Laboratory of Quality Research in Chinese Medicine, and Institute of Chinese Medical Sciences, University of Macau, Macau SAR, China.
Int J Biol Macromol. 2015 Aug;79:180-5. doi: 10.1016/j.ijbiomac.2015.04.040. Epub 2015 Apr 30.
The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID). Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively. Furthermore, bioactive polysaccharides from the mycelium of C. sinensis fungus UM01 were determined as polysaccharide fractions with the molecular weight above 10 kDa. The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID. Results are helpful to establish an efficient and controllable fermentation process for the industrial production of bioactive polysaccharides from C. sinensis UM01, and beneficial to develop a unique health and functional product in future.
采用正交设计以及高效体积排阻色谱结合多角度激光光散射和示差折光检测器（HPSEC-MALLS-RID），研究了中华虫草菌UM01菌丝体产生活性多糖的最佳发酵条件和培养基。结果表明，菌丝体生长的最佳温度、初始pH值、转速、培养基装量（培养基体积与三角瓶体积之比）和接种量分别为15℃、pH 6.0、150 rpm、2/5（v/v）和3%（v/v）。此外，中华虫草菌UM01菌丝体的活性多糖被确定为分子量大于10 kDa的多糖组分。根据HPSEC-MALLS/RID测定的活性多糖最大量（486.16±19.60 mg/L），确定最佳发酵培养基组成为葡萄糖30.0 g/L、蔗糖30.0 g/L、KH2PO4 1.0 g/L、CaCl2 0.5 g/L、酵母提取物3.0 g/L和MgCl2 0.1 g/L。这些结果有助于建立一个高效可控的发酵工艺，用于从中华虫草UM01工业化生产活性多糖，并且有利于未来开发独特的健康功能性产品。
