Karunakaran Karuna P, Yu Hong, Foster Leonard J, Brunham Robert C
Vaccine Research Laboratory, UBC Centre for Disease Control, University of British Columbia, 655 West 12th Avenue, Vancouver, BC, Canada, V5Z 4R4.
Department of Biochemistry and Molecular Biology, Centre for High-Throughput Biology, University of British Columbia, Vancouver, BC, Canada.
Methods Mol Biol. 2016;1403:419-32. doi: 10.1007/978-1-4939-3387-7_23.
Vaccines based on humoral immunity alone are unlikely to protect against infections caused by intracellular pathogens and today's most pressing infectious diseases of public health importance are caused by intracellular infections that include tuberculosis, malaria, HIV/AIDS, and others such as Chlamydia trachomatis. For these infections, vaccines that induce cellular immune responses are essential. Major impediments in developing such vaccines include difficulty in identifying relevant T cell antigens and delivering them in ways that elicit protective cellular immunity. Genomics and proteomics now provide tools to allow unbiased empirical identification of candidate T cell antigens. This approach represents an advance on bioinformatic searches for candidate T cell antigens. This chapter discusses an immunoproteomic approach we have used to identify Chlamydia T cell antigens. We further discuss how these T cell antigens can be developed into a human vaccine.
仅基于体液免疫的疫苗不太可能预防由细胞内病原体引起的感染，而当今公共卫生领域最紧迫的重要传染病均由细胞内感染所致，包括结核病、疟疾、艾滋病毒/艾滋病，以及其他如沙眼衣原体等。对于这些感染，诱导细胞免疫反应的疫苗至关重要。开发此类疫苗的主要障碍包括难以识别相关的T细胞抗原，以及难以以引发保护性细胞免疫的方式递送这些抗原。基因组学和蛋白质组学现在提供了工具，可用于无偏见地经验性鉴定候选T细胞抗原。这种方法是对候选T细胞抗原进行生物信息学搜索的一种进步。本章讨论了我们用于鉴定衣原体T细胞抗原的免疫蛋白质组学方法。我们还将进一步讨论如何将这些T细胞抗原开发成人类疫苗。
