Frey Joachim, Burr Sarah E, Wahli Thomas
Institute of Veterinary Bacteriology, Vetsuisse, University of Bern, Laenggassstrasse 122, 3001, Bern, Switzerland.
Clinical Research Department, London School of Hygiene and Tropical Medicine, London, UK.
Methods Mol Biol. 2016;1404:203-209. doi: 10.1007/978-1-4939-3389-1_14.
The production of an immersion vaccine and the vaccination procedure to immunize fry of perch (Perca fluviatilis L.) against pathogenic Aeromonas sobria that harbor a type III secretion system is described. The vaccine, based on chemically inactivated A. sobria, enables rapid vaccination of a large number of fish by immersion of fry in an aqueous vaccine suspension during 5 min, giving them high protection during fattening under open water conditions in a freshwater lake for at least 4 months.
本文描述了一种浸泡疫苗的生产方法以及用于免疫河鲈（Perca fluviatilis L.）鱼苗以抵抗携带III型分泌系统的致病性温和气单胞菌的接种程序。该疫苗基于化学灭活的温和气单胞菌，通过将鱼苗在疫苗水悬浮液中浸泡5分钟，能够快速对大量鱼苗进行接种，使其在淡水湖开放水域条件下育肥期间获得至少4个月的高度保护。
