Rupp Melanie, Pilo Paola, Müller Barbara, Knüsel Ralph, von Siebenthal Beat, Frey Joachim, Sindilariu Paul-Daniel, Schmidt-Posthaus Heike
Centre for Fish and Wildlife Health, University of Bern, Bern, Switzerland.
Institute of Veterinary Bacteriology, University of Bern, Bern, Switzerland.
J Fish Dis. 2019 May;42(5):685-691. doi: 10.1111/jfd.12970. Epub 2019 Feb 26.
In non-salmonid fish, Aeromonas salmonicidacan cause local infections with severe skin ulcerations, known as atypical furunculosis. In this study, we present a systemic infection by a virulent A. salmonicidain European perch (Perca fluviatilis).This infection was diagnosed in a Swiss warm water recirculation aquaculture system. The isolate of A. salmonicida encodes a type three secretion system (TTSS) most likely located on a plasmid similar to pAsa5/pASvirA, which is known to specify one of the main virulence attributes of the species A. salmonicida. However, the genes specifying the TTSS of the perch isolate show a higher temperature tolerance than strains isolated from cold-water fish. The function of the TTSS in virulence was verified in a cytotoxicity test using bluegill fry and epithelioma papulosum cyprinid cells.
在非鲑科鱼类中，杀鲑气单胞菌可引起局部感染，并伴有严重的皮肤溃疡，即所谓的非典型疖疮病。在本研究中，我们展示了欧洲鲈鱼（河鲈）被强毒性杀鲑气单胞菌引起的全身性感染。这种感染是在瑞士的一个温水循环水产养殖系统中诊断出来的。杀鲑气单胞菌的分离株编码一种三型分泌系统（TTSS），该系统很可能位于与pAsa5/pASvirA相似的质粒上，已知该质粒决定了杀鲑气单胞菌的一种主要毒力特性。然而，鲈鱼分离株中决定TTSS的基因比从冷水鱼中分离出的菌株表现出更高的温度耐受性。在使用蓝鳃太阳鱼幼鱼和鲤上皮瘤细胞进行的细胞毒性试验中，验证了TTSS在毒力方面的作用。
