Shibasaki Seiji, Ueda Mitsuyoshi
General Education Center and Graduate School of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo-ku, Kobe, 650-8530, Japan.
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
Methods Mol Biol. 2016;1404:497-509. doi: 10.1007/978-1-4939-3389-1_32.
Oral vaccines are easier to administer than injectable vaccines. To induce an adequate immune response using vaccines, antigenic proteins are usually combined with adjuvant materials. This chapter presents methodologies for the design of oral vaccines using molecular display technology. In molecular display technology, antigenic proteins are displayed on a microbial cell surface with adjuvant ability. This technology would provide a quite convenient process to produce oral vaccines when the DNA sequence of an efficient antigenic protein is available. As an example, oral vaccines against candidiasis were introduced using two different molecular display systems with Saccharomyces cerevisiae and Lactobacillus casei.
口服疫苗比注射用疫苗更容易给药。为了使用疫苗诱导足够的免疫反应，抗原蛋白通常与佐剂材料结合。本章介绍了使用分子展示技术设计口服疫苗的方法。在分子展示技术中，抗原蛋白展示在具有佐剂能力的微生物细胞表面。当有效抗原蛋白的DNA序列可用时，该技术将为生产口服疫苗提供一个非常方便的过程。例如，使用酿酒酵母和干酪乳杆菌的两种不同分子展示系统介绍了抗念珠菌病的口服疫苗。
