Shibasaki Seiji, Karasaki Miki, Tafuku Senji, Aoki Wataru, Sewaki Tomomitsu, Ueda Mitsuyoshi
General Education Center and Graduate School of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo-ku, Kobe 650-8530, Japan.
Genolac BL Corporation, Okinawa Industry Support Center 4F, 1831-1, Oroku, Naha City, Okinawa 901-0152, Japan.
Sci Pharm. 2014 Jul 23;82(3):697-708. doi: 10.3797/scipharm.1404-07. Print 2014 Jul-Sep.
Candidiasis is a common fungal infection that is prevalent in immunocompromised individuals. In this study, an oral vaccine against Candida albicans was developed by using the molecular display approach. Enolase 1 protein (Eno1p) of C. albicans was expressed on the Lactobacillus casei cell surface by using poly-gamma-glutamic acid synthetase complex A from Bacillus subtilis as an anchoring protein. The Eno1p-displaying L. casei cells were used to immunize mice, which were later challenged with a lethal dose of C. albicans. The data indicated that the vaccine elicited a strong IgG response and increased the survival rate of the vaccinated mice. Furthermore, L. casei acted as a potent adjuvant and induced high antibody titers that were comparable to those induced by strong adjuvants such as the cholera toxin. Overall, the molecular display method can be used to rapidly develop vaccines that can be conveniently administered and require minimal processing.
念珠菌病是一种常见的真菌感染，在免疫功能低下的个体中普遍存在。在本研究中，采用分子展示方法开发了一种针对白色念珠菌的口服疫苗。通过使用来自枯草芽孢杆菌的聚γ-谷氨酸合成酶复合体A作为锚定蛋白，将白色念珠菌的烯醇化酶1蛋白（Eno1p）表达在干酪乳杆菌细胞表面。用展示Eno1p的干酪乳杆菌细胞免疫小鼠，随后用致死剂量的白色念珠菌对其进行攻击。数据表明，该疫苗引发了强烈的IgG反应，并提高了接种疫苗小鼠的存活率。此外，干酪乳杆菌作为一种有效的佐剂，诱导产生的高抗体滴度与霍乱毒素等强佐剂诱导产生的抗体滴度相当。总体而言，分子展示方法可用于快速开发易于给药且所需加工最少的疫苗。
