长链非编码RNA LOC101926975的表达下调损害细胞增殖和细胞周期及其在先天性巨结肠症患者中的临床意义
Downregulated Expression of Long Non-Coding RNA LOC101926975 Impairs both Cell Proliferation and Cell Cycle and Its Clinical Implication in Hirschsprung Disease Patients.
作者信息
Shen Ziyang, Peng Lei, Zhu Zhongxian, Xie Hua, Zang Rujin, Du Chunxia, Chen Guanglin, Li Hongxing, Xia Yankai, Tang Weibing
机构信息
1. Department of Pediatric Surgery, Nanjing Children's Hospital Affiliated Nanjing Medical University, Nanjing 210008; 2. State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
2. State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 211166, China; 3. Key Laboratory of Modern Toxicology (Nanjing Medical University), Ministry of Education, China.
出版信息
Int J Med Sci. 2016 Apr 8;13(4):292-7. doi: 10.7150/ijms.14187. eCollection 2016.
BACKGROUND
Long non-coding RNAs (lncRNAs) have been reported to participate in various diseases. Hirschsprung disease (HSCR) is a common digestive disease in the new born. However, the relationship between lncRNAs and HSCR remains unclarified.
METHODS
We used qRT-PCR to detect the relative expression of LOC101926975 in 80 pairs of HSCR bowel tissues and matched normal bowel tissues. CCK-8 assay, transwell assay and flow cytometry were then used to evaluate the function in vitro by knocking down the LOC101926975 in SK-N-BE(2) cells. Receiver operating characteristic (ROC) curve was used to evaluate the potential diagnostic value of LOC101926975.
RESULTS
LOC101926975 was significantly downregulated in HSCR tissues with excellent correlation with FGF1. Dysregulation of LOC101926975 suppressed cell proliferation and induced G0/G1 arrest without impact on cell apoptosis or migration. Meanwhile, the AUC of LOC101926975 was 0.900 which presented great diagnostic value.
CONCLUSIONS
Our study firstly investigates the potential function of LOC101926975 in HSCR and infers that LOC101926975 can distinguish HSCR from the normal ones.
背景
长链非编码RNA（lncRNAs）已被报道参与多种疾病。先天性巨结肠（HSCR）是新生儿常见的消化系统疾病。然而，lncRNAs与HSCR之间的关系仍不明确。
方法
我们使用qRT-PCR检测80对HSCR肠组织和配对的正常肠组织中LOC101926975的相对表达。然后通过敲低SK-N-BE(2)细胞中的LOC101926975，使用CCK-8检测、Transwell检测和流式细胞术评估其体外功能。采用受试者工作特征（ROC）曲线评估LOC101926975的潜在诊断价值。
结果
LOC101926975在HSCR组织中显著下调，与FGF1具有良好的相关性。LOC101926975的失调抑制细胞增殖并诱导G0/G1期阻滞，而不影响细胞凋亡或迁移。同时，LOC101926975的AUC为0.900，具有很大的诊断价值。
结论
我们的研究首次探讨了LOC101926975在HSCR中的潜在功能，并推断LOC101926975可将HSCR与正常情况区分开来。
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