Hakimuddin F, Abidi F, Jafer O, Li C, Wernery U, Hebel Ch, Khazanehdari K
Molecular Biology and Genetics Laboratory, P.O. Box 597, Dubai, United Arab Emirates.
Central Veterinary Research Laboratory, P.O. Box 597, Dubai, United Arab Emirates.
Biomol Detect Quantif. 2015 Nov 11;6:27-32. doi: 10.1016/j.bdq.2015.10.001. eCollection 2016 Jan.
Beak and feather disease is caused by Circovirus, which affects actively growing beak and feather cells of avian species. The disease affects mainly young birds while older birds may overcome the disease with few lasting effects. Due to lack of treatment, the only way to control the disease is through hygiene and early diagnosis. As a diagnostic tool, we have established a Taqman probe based real-time PCR assay to detect the presence of the viral genome in psittacine birds in UAE and reported the incidence of circovirus in different species of psittacine birds. The sensitivity of our assay was found to be very high with detection limit of up to 3.5 fg of DNA in the sample. The mean prevalence of circovirus was found to be 58.33% in African Grey Parrots, 34.42% in Cockatoos, 31.8% in amazon parrots and 25.53% in Macaws. The Taqman assay is a quick, reliable and sensitive detection method that has been instrumental in identifying this disease that was not previously reported in the region.
喙羽病由圆环病毒引起,该病毒会影响鸟类正在生长的喙部和羽毛细胞。这种疾病主要影响幼鸟,而成年鸟可能会战胜疾病,且几乎不会留下持久影响。由于缺乏治疗方法,控制该疾病的唯一途径是保持卫生和进行早期诊断。作为一种诊断工具,我们建立了一种基于Taqman探针的实时PCR检测方法,以检测阿联酋鹦鹉体内病毒基因组的存在,并报告了不同种类鹦鹉中圆环病毒的发病率。我们的检测方法灵敏度非常高,样本中DNA的检测限高达3.5 fg。发现圆环病毒在非洲灰鹦鹉中的平均患病率为58.33%,在凤头鹦鹉中为34.42%,在亚马逊鹦鹉中为31.8%,在金刚鹦鹉中为25.53%。Taqman检测方法是一种快速、可靠且灵敏的检测方法,在识别该地区此前未报告的这种疾病方面发挥了重要作用。