Haddadmarandi M R, Madani S A, Nili H, Ghorbani A
Resident of Avian Disease, Department of Avian Medicine, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Iran J Vet Res. 2018 Winter;19(1):22-26.
Beak and feather disease virus (BFDV), a member of genus circovirus, is a small, non-enveloped, single stranded DNA virus. Although BFDVs are among the most well studied circoviruses, there is little to no information about BFDVs in Iran. The aim of the present study was to detect and identify BFDV molecules from the birds referred to the avian clinic of The Faculty of Veterinary Medicine, Tehran University, Iran. A total of 55 DNA samples were extracted from birds from nine different species of the order psittaciformes. A robust conventional polymerase chain reaction (PCR) was applied to detect the gene of the virus. Ten out of 55 samples, from four different species, were tested positive for BFDVs in PCR ( (4), (3), (2), (1)). Molecular identification of the detected BFDVs was performed based on their gene sequences. The phylogenetic analysis revealed that the Iranian BFDVs from this study were clustered into four genetically distinct clades belonging to different genetic subtypes of BFDVs (L1, N1, T1, and I4). Although the relation between the samples and their related subtypes in the tree are discussed, further studies are needed to elucidate the host specificity and incidence of the BFDVs from different genetic subtypes.
喙羽病病毒（BFDV）是圆环病毒属的成员，是一种小型、无包膜的单链DNA病毒。尽管BFDV是研究最为深入的圆环病毒之一，但在伊朗，关于BFDV的信息却很少甚至几乎没有。本研究的目的是从转诊至伊朗德黑兰大学兽医学院鸟类诊所的鸟类中检测和鉴定BFDV分子。从鹦形目九个不同物种的鸟类中总共提取了55份DNA样本。应用一种可靠的常规聚合酶链反应（PCR）来检测该病毒的基因。在55份样本中，有10份来自四个不同物种的样本在PCR检测中呈BFDV阳性（（4），（3），（2），（1））。基于检测到的BFDV的基因序列进行分子鉴定。系统发育分析表明，本研究中的伊朗BFDV聚集在四个遗传上不同的进化枝中，属于BFDV的不同遗传亚型（L1、N1、T1和I4）。尽管在树形图中讨论了样本与其相关亚型之间的关系，但仍需要进一步研究以阐明不同遗传亚型BFDV的宿主特异性和发病率。
