Hameed Dharjath S, Sapmaz Aysegul, Ovaa Huib
Division of Cell Biology II, The Netherlands Cancer Institute , Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.
Department of Chemical Immunology, Leiden University Medical Center , Einthovenweg 20, 2333 ZC Leiden, The Netherlands.
Bioconjug Chem. 2017 Mar 15;28(3):805-815. doi: 10.1021/acs.bioconjchem.6b00140. Epub 2016 Apr 28.
Ubiquitin (Ub) is a small post-translational modifier protein involved in a myriad of biochemical processes including DNA damage repair, proteasomal proteolysis, and cell cycle control. Ubiquitin signaling pathways have not been completely deciphered due to the complex nature of the enzymes involved in ubiquitin conjugation and deconjugation. Hence, probes and assay reagents are important to get a better understanding of this pathway. Recently, improvements have been made in synthesis procedures of Ub derivatives. In this perspective, we explain various research reagents available and how chemical synthesis has made an important contribution to Ub research.
泛素（Ub）是一种小的翻译后修饰蛋白，参与众多生化过程，包括DNA损伤修复、蛋白酶体蛋白水解和细胞周期控制。由于参与泛素缀合和解缀合的酶的复杂性质，泛素信号通路尚未完全破译。因此，探针和检测试剂对于更好地理解这一通路很重要。最近，泛素衍生物的合成程序有了改进。从这个角度出发，我们解释了各种可用的研究试剂，以及化学合成如何对泛素研究做出了重要贡献。
