Sun Xiaoyu, Liu Bin, Chen Yan, Huang Honglan, Wang Guoqing, Li Fan, Ni Zhaohui
a Key Laboratory of Zoonosis, Ministry of Education, Department of Pathogenobiology , College of Basic Medical Science, Jilin University , Changchun , P.R. China.
b Cardiovascular Disease Center, The First Hospital of Jilin University , Changchun , P.R. China.
J Chemother. 2016 Dec;28(6):469-475. doi: 10.1080/1120009X.2015.1133014. Epub 2016 Feb 6.
The prevalence of various Ambler class A to D β-lactamases, ISAba1, and class 1 and 2 integrons as well as the clonal relatedness in 105 Acinetobacter spp. isolates found in northeastern China was investigated. All 105 Acinetobacter spp. isolates were determined to be multidrug resistant (MDR), and the resistance rates to carbapenem agents were approximately 50%. PER, IMP, AmpC, and OXA-23 were found to be dominant β-lactamases belonging to different classes, respectively. This is the first report of the coexistence of bla, bla, bla, and bla genes in Acinetobacter spp. isolates from northeastern China. ISAba1 was found upstream of the bla gene in 87.8% (36/41) strains and upstream of the bla gene in 26.5% (13/49) strains. ISAba3-like element was found upstream of the bla gene in one bla-positive strain. The presence of IntI1 was detected in 63.8% (67/105) of the isolates and the most prevalent gene cassettes were aacA4, aadA1, and catB8. The highly prevalent isolates belong to international clonal lineage (ICL)-II. These results indicate that the wide horizontal and clonal spread of MDR Acinetobacter spp. isolates harbouring multiple β-lactamase genes has become a serious problem in northeastern China.
对在中国东北地区发现的105株不动杆菌属菌株中各种Ambler A至D类β-内酰胺酶、ISAba1以及1类和2类整合子的流行情况以及克隆相关性进行了调查。所有105株不动杆菌属菌株均被确定为多重耐药（MDR），对碳青霉烯类药物的耐药率约为50%。发现PER、IMP、AmpC和OXA-23分别是属于不同类别的主要β-内酰胺酶。这是中国东北地区不动杆菌属菌株中bla、bla、bla和bla基因共存的首次报道。在87.8%（36/41）的菌株中，ISAba1位于bla基因上游，在26.5%（13/49）的菌株中位于bla基因上游。在一株bla阳性菌株中，在bla基因上游发现了ISAba3样元件。在63.8%（67/105）的分离株中检测到IntI1的存在，最常见的基因盒是aacA4、aadA1和catB8。高度流行的分离株属于国际克隆谱系（ICL）-II。这些结果表明，携带多种β-内酰胺酶基因的多重耐药不动杆菌属菌株的广泛水平传播和克隆传播已成为中国东北地区的一个严重问题。
