一种结合了成骨细胞和破骨细胞的 3D 血管化骨重塑模型，该模型存在于富含 CaP 纳米颗粒的基质中。
A 3D vascularized bone remodeling model combining osteoblasts and osteoclasts in a CaP nanoparticle-enriched matrix.
机构信息
Cell and Tissue Engineering Laboratory, IRCCS Galeazzi Orthopaedic Institute, 20161 Milan, Italy.
PhD School in Life Sciences, Department of Biotechnology & Biosciences, University of Milano-Bicocca, 20126 Milan, Italy.
出版信息
Nanomedicine (Lond). 2016 May;11(9):1073-91. doi: 10.2217/nnm-2015-0021. Epub 2016 Apr 14.
AIM
We aimed to establish a 3D vascularized in vitro bone remodeling model.
MATERIALS & METHODS: Human umbilical endothelial cells (HUVECs), bone marrow mesenchymal stem cells (BMSCs), and osteoblast (OBs) and osteoclast (OCs) precursors were embedded in collagen/fibrin hydrogels enriched with calcium phosphate nanoparticles (CaPn). We assessed vasculogenesis in HUVEC-BMSC coculture, osteogenesis with OBs, osteoclastogenesis with OCs, and, ultimately, cell interplay in tetraculture.
RESULTS
HUVECs developed a robust microvascular network and BMSCs differentiated into mural cells. Noteworthy, OB and OC differentiation was increased by their reciprocal coculture and by CaPn, and even more by the combination of the tetraculture and CaPn.
CONCLUSION
We successfully developed a vascularized 3D bone remodeling model, whereby cells interacted and exerted their specific function.
目的
我们旨在建立一个 3D 血管化的体外骨重塑模型。
材料与方法
将人脐静脉内皮细胞（HUVEC）、骨髓间充质干细胞（BMSC）以及成骨细胞（OB）和破骨细胞（OC）前体细胞嵌入富含磷酸钙纳米颗粒（CaPn）的胶原/纤维蛋白水凝胶中。我们评估了 HUVEC-BMSC 共培养中的血管生成、OB 中的成骨作用、OC 中的破骨作用以及最终的四细胞共培养中的细胞相互作用。
结果
HUVEC 形成了强大的微血管网络，BMSC 分化为壁细胞。值得注意的是，OB 和 OC 的分化通过它们的相互共培养以及 CaPn 得到增强，而通过四细胞共培养和 CaPn 的组合增强得更多。
结论
我们成功地开发了一个血管化的 3D 骨重塑模型，其中细胞相互作用并发挥其特定功能。
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