Fabres Laura Fuhrich, Rosa Dos Santos Sayonara Peixoto, Benitez Lisianne Brittes, Rott Marilise Brittes
Acta Parasitol. 2016 Mar;61(2):221-7. doi: 10.1515/ap-2016-0031.
Free-living amoebae (FLA) are widely distributed in soil and water. A few number of them are implicated in human disease: Acanthamoeba spp., Naegleria fowleri, Balamuthia mandrillaris and Sappinia diploidea. Species of Acanthamoeba can cause keratitis and brain infections. In this study, 72 water samples were taken from both hot tubs and thermal swimming pools in the city of Porto Alegre, RS, Brazil, to determine the presence of Acanthamoeba in the water as well as perform the phenotypic and genotypic characterization of the isolates. The identification of the isolates was based on the cysts morphology and PCR amplification using genus-specific oligonucleotides. When the isolates were submitted to PCR reaction only 8 were confirmed as belonging to the genus Acanthamoeba. The sequences analysis when compared to the sequences in the GenBank, showed genotype distribution in group T3 (12,5%), T5 (12,5%), T4 (25%) and T15 (50%). The results of this study confirmed the presence of potentially pathogenic isolates of free living amoebae in hot swimming pool and spas which can present risks to human health.
自由生活阿米巴（FLA）广泛分布于土壤和水中。其中少数与人类疾病有关：棘阿米巴属、福氏耐格里阿米巴、曼氏巴罗孢虫和双相萨平虫。棘阿米巴属物种可引起角膜炎和脑部感染。在本研究中，从巴西里约热内卢州阿雷格里港的热水浴缸和温水游泳池采集了72份水样，以确定水中棘阿米巴的存在情况，并对分离株进行表型和基因型特征分析。分离株的鉴定基于囊肿形态和使用属特异性寡核苷酸的PCR扩增。当分离株进行PCR反应时，只有8株被确认为属于棘阿米巴属。与GenBank中的序列进行比较时，序列分析显示基因型分布在T3组（12.5%）、T5组（12.5%）、T4组（25%）和T15组（50%）。本研究结果证实，在热水游泳池和温泉中存在自由生活阿米巴的潜在致病分离株，这可能对人类健康构成风险。
