Dayawansa Samantha, Zhang Jun, Shih Chung-Hsuan, Tharakan Binu, Huang Jason H
a Department of Neurosurgery , Baylor Scott & White Healthcare , Temple , Texas , USA.
b Department of Surgery , Texas A&M Health Science Center College of Medicine , Temple , Texas , USA.
Neurol Res. 2016 Apr;38(4):352-7. doi: 10.1080/01616412.2015.1105586. Epub 2016 Mar 28.
Functional data are essential when confirming the efficacy of elongated dorsal root ganglia (DRG) cells as a substitute for autografting. We present the quantitative functional motor, electrophysiological findings of engineered DRG recipients for the first time.
Elongated DRG neurons and autografts were transplanted to bridge 1-cm sciatic nerve lesions of Sprague Dawley (SD) rats. Motor recoveries of elongated DRG recipients (n=9), autograft recipients (n=9), unrepaired rats (n=9) and intact rats (n=6) were investigated using the angle board challenge test following 16 weeks of recovery. Electrophysiology studies were conducted to assess the functional recovery at 16 weeks. In addition, elongated DRGs were subjected to histology assessments.
At threshold levels (35° angle) of the angle board challenge test, the autograft recipients', DRG recipients' and unrepaired group's performances were equal to each other and were less than the intact group (p<0.05). However, during the subthreshold (30°) angle board challenge test, the elongated DRG recipients' performance was similar to both the intact group and the autograft nerve recipients, and was better (p<0.05) than the unrepaired group. The autograft recipients' performance was similar to the unrepaired group and was significantly different (p<0.05) compared with the performance of the intact group. During electrophysiological testing, the rats with transplanted engineered DRG constructs had intact signal transmission when recorded over the lesion, while the unrepaired rats did not. It was observed that elongated DRG neurons closely resembled an autograft during histological assessments.
Performances of autograft and elongated DRG construct recipients were similar. Elongated DRG neurons should be further investigated as a substitute for autografting.
在确认延长背根神经节（DRG）细胞作为自体移植替代物的疗效时，功能数据至关重要。我们首次展示了经工程改造的DRG受体的定量功能运动和电生理研究结果。
将延长的DRG神经元和自体移植物移植到Sprague Dawley（SD）大鼠1厘米坐骨神经损伤处以进行桥接。在恢复16周后，使用角板挑战试验研究延长的DRG受体（n = 9）、自体移植受体（n = 9）、未修复大鼠（n = 9）和完整大鼠（n = 6）的运动恢复情况。进行电生理研究以评估16周时的功能恢复情况。此外，对延长的DRG进行组织学评估。
在角板挑战试验的阈值水平（35°角）下，自体移植受体、DRG受体和未修复组的表现彼此相当，且均低于完整组（p<0.05）。然而，在亚阈值（30°）角板挑战试验中，延长的DRG受体的表现与完整组和自体移植神经受体相似，且优于未修复组（p<0.05）。自体移植受体的表现与未修复组相似，与完整组的表现相比有显著差异（p<0.05）。在电生理测试中，移植了经工程改造的DRG构建体的大鼠在损伤部位记录时有完整的信号传导，而未修复的大鼠则没有。在组织学评估中观察到延长的DRG神经元与自体移植物非常相似。
自体移植和延长的DRG构建体受体的表现相似。延长的DRG神经元应作为自体移植的替代物进行进一步研究。
