Matsson P, Almlöf I, Nilsson K, Ahlstedt S
Pharmacia Diagnostics, Uppsala University, Sweden.
Int Arch Allergy Appl Immunol. 1989;88(1-2):122-5. doi: 10.1159/000234762.
The basophilic propagating activity was determined in conditioned media obtained from cell cultures of mononuclear cells from atopic and healthy individuals. The activity was analyzed as the capacity to induce differentiation in the human basophilic cell line KU812. The KU812 cells responded primarily to cultured media with histamine production and secondarily with granulation. Eight atopic individuals, 3 of whom having mild symptoms, with known birch allergies were selected together with 3 control individuals. Total and specific IgE were analyzed in sera, and basophil and eosinophil counts were determined from blood smears after Wright's staining. Significant differences between the symptomatic atopic individuals and the control group were obtained for eosinophil counts (p less than 0.05) and in the KU812 basophilic differentiation activity assay (p less than 0.01). No single test could significantly discriminate between symptomatic and nonsymptomatic individuals, but the KU812 cell assay in combination with the eosinophil count clearly distinguished the symptomatic individuals from the control group. Increased levels of IgE specific to birch allergen were only obtained in the atopic individuals (p less than 0.001). No difference in basophilic propagating activity was observed with supernatants from cells incubated with birch pollen allergen. Serum tested for basophilic propagating activity showed no or decreased values of inducing histamine production in the KU812 cell line. In conclusion, the KU812 assay for basophilic propagating activity was the most useful discriminating test to select symptomatic atopic individuals from nonsymptomatic atopic and control individuals.
在从特应性个体和健康个体的单核细胞的细胞培养物中获得的条件培养基中测定嗜碱性粒细胞增殖活性。该活性被分析为诱导人嗜碱性细胞系KU812分化的能力。KU812细胞主要对培养介质产生组胺作出反应，其次是颗粒化。选择了8名已知对桦树过敏的特应性个体，其中3人有轻度症状，以及3名对照个体。分析血清中的总IgE和特异性IgE，并通过瑞氏染色后从血涂片测定嗜碱性粒细胞和嗜酸性粒细胞计数。在嗜酸性粒细胞计数（p小于0.05）和KU812嗜碱性粒细胞分化活性测定（p小于0.01）方面，有症状的特应性个体与对照组之间存在显著差异。没有单一测试能够显著区分有症状和无症状个体，但KU812细胞测定与嗜酸性粒细胞计数相结合能够清楚地区分有症状个体与对照组。仅在特应性个体中获得了针对桦树过敏原的IgE水平升高（p小于0.001）。用桦树花粉过敏原孵育的细胞上清液未观察到嗜碱性粒细胞增殖活性的差异。测试嗜碱性粒细胞增殖活性的血清在KU812细胞系中显示无诱导组胺产生的值或值降低。总之，用于嗜碱性粒细胞增殖活性的KU812测定是从无症状特应性个体和对照个体中选择有症状特应性个体的最有用的鉴别测试。
