Mencia-Huerta J M, Touvay C, Pfister A, Braquet P
Institut Henri Beaufour, Département d'Immunologie, Les Ulis, France.
Int Arch Allergy Appl Immunol. 1989;88(1-2):154-6. doi: 10.1159/000234771.
To further substantiate the possible role of platelet-activating factor (PAF) in the development of bronchial hyperreactivity, Alzet osmotic minipumps loaded with the autacoid or solvent alone were placed under the back skin of male Hartley guinea pigs and connected to the jugular vein for 15 days. As compared to the animals treated with solvent alone, guinea pigs receiving PAF (20 micrograms/kg/h, 15 days) exhibited a hyperresponsiveness to histamine that was not observed in those simultaneously treated with PAF and the PAF antagonist BN 52021. Lungs from PAF-treated animals were congestive in appearance, bronchi and bronchioli were contracted, Reisseisen muscles were markedly hypertrophied, and a muciparous metaplasia of the endothelium was observed. The number of eosinophils was significantly increased in the parenchyma, as was the number of mast cells in the peribronchial zones in the lungs from guinea pigs chronically treated with PAF. Lungs from control animals did not show morphologic alterations or eosinophil and mast cell infiltration. These data indicate that long-term treatment with PAF induces profound alterations in the respiratory system, resembling those observed in asthmatic patients. This result is in keeping with the possible role of this autacoid in the development of bronchial hyperreactivity.
为了进一步证实血小板活化因子（PAF）在支气管高反应性发展过程中可能发挥的作用，将装有该自体活性物质或仅装溶剂的Alzet渗透微型泵置于雄性Hartley豚鼠的背部皮肤下，并与颈静脉相连，持续15天。与仅用溶剂处理的动物相比，接受PAF（20微克/千克/小时，持续15天）的豚鼠对组胺表现出高反应性，而在同时接受PAF和PAF拮抗剂BN 52021处理的豚鼠中未观察到这种情况。接受PAF处理的动物的肺外观充血，支气管和细支气管收缩，Reisseisen肌明显肥大，并且观察到内皮细胞出现黏液化生。长期接受PAF处理的豚鼠肺实质中的嗜酸性粒细胞数量显著增加，肺支气管周围区域的肥大细胞数量也显著增加。对照动物的肺未显示形态学改变或嗜酸性粒细胞和肥大细胞浸润。这些数据表明，长期用PAF处理会引起呼吸系统的深刻改变，类似于在哮喘患者中观察到的情况。这一结果与这种自体活性物质在支气管高反应性发展中可能发挥的作用相符。
