[五味子提取物对暴露于苯并[a]芘的早期妊娠大鼠胚胎毒性和生殖毒性的保护作用]
[Protective Effect of Schisandra Extract on Embryotoxicity and Reproductive Toxicity in Early Pregnant Rats Exposed to Benzo [a] pyrene].
作者信息
Liang Jing, Hou Hai-yan, Sun Yang, Chen Ya-qiong
出版信息
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2016 Feb;36(2):234-8.
OBJECTIVE
To observe protective effects of Schisandra extract (SE) on embryotoxicity and reproductive toxicity of early pregnant rats exposed to Benzo[a]pyrene (Bap).
METHODS
Pregnant rat model was prepared using periodic screening cage method. Totally 50 female pregnant SD rats were divided into five groups by randomized block design according to the weight, i.e., the BaP model group, the low dose SE group, the middle dose SE group, the high dose SE group, the normal control group, 10 rats in each group. Rats in the BaP model group were administered with BaP at a daily dose of 2 mg/kg by gastrogavage. Rats in low, middle, and high dose SE groups were administered by gastrogavage with BaP (at a daily dose of 2 mg/kg) plus SE at a daily dose of 40, 200, and 1 000 mg/kg, respectively. Equal volume of olive oil was administered to rats in the normal control group by gastrogavage. All medication was performed for 8 successive days. Changes of rat body weight in each period were observed. The uterus embryonic total quality and ovary quality were measured, and organ index calculated. The number of corpus luteum, the number of embryo implantation, and the number of absorbed embryo were statistically calculated respectively. The implantation rate and the absorbed embryos rate were calculated. Serum levels of human chorionic gonadotrophin β (β-HCG) and progesterone (PROG) were detected by ELISA.
RESULTS
Compared with the normal control group, the weight of 9-day pregnant rats, the number of embryo implantation, the uterus embryonic total index, ovary index, serum levels of β-HCG and PROG all decreased in the Bap model group with significant difference (P < 0.05, P < 0.01). Compared with the Bap model group, body weight, the uterus embryonic total index, and the PROG level increased in 3 dose SE groups (P < 0.05, P < 0.01). Ovary index and serum β-HCG increased in middle and high dose SE groups (P < 0.05, P < 0.01). The number of implantation obviously increased in the high dose SE groups (P < 0.01).
CONCLUSION
SE could reduce the embryotoxicity and reproductive toxicity of early pregnant rats exposed to Benzo[a]pyrene.
目的
观察五味子提取物（SE）对暴露于苯并[a]芘（Bap）的早期妊娠大鼠胚胎毒性和生殖毒性的保护作用。
方法
采用定期筛选笼法制备妊娠大鼠模型。将50只雌性妊娠SD大鼠按体重随机区组设计分为5组，即Bap模型组、低剂量SE组、中剂量SE组、高剂量SE组、正常对照组，每组10只。Bap模型组大鼠每日按2 mg/kg剂量灌胃给予Bap。低、中、高剂量SE组大鼠每日按2 mg/kg剂量灌胃给予Bap加40、200、1 000 mg/kg剂量的SE。正常对照组大鼠灌胃给予等体积的橄榄油。连续给药8天。观察各时期大鼠体重变化。测量子宫胚胎总质量和卵巢质量，并计算器官指数。分别统计黄体数、胚胎着床数和吸收胚胎数。计算着床率和吸收胚胎率。采用酶联免疫吸附测定法（ELISA）检测血清人绒毛膜促性腺激素β（β-HCG）和孕酮（PROG）水平。
结果
与正常对照组相比，Bap模型组妊娠9天大鼠体重、胚胎着床数、子宫胚胎总指数、卵巢指数、血清β-HCG和PROG水平均降低，差异有统计学意义（P<0.05，P<0.01）。与Bap模型组相比,3个剂量SE组大鼠体重、子宫胚胎总指数和PROG水平升高（P<0.05，P<0.01）。中、高剂量SE组卵巢指数和血清β-HCG升高（P<0.05，P<0.01）。高剂量SE组着床数明显增加（P<0.01）。
结论
SE可降低暴露于苯并[a]芘的早期妊娠大鼠的胚胎毒性和生殖毒性。
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