• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

垂体前叶发育和催乳素细胞分化需要ZBTB20。

ZBTB20 is required for anterior pituitary development and lactotrope specification.

作者信息

Cao Dongmei, Ma Xianhua, Cai Jiao, Luan Jing, Liu An-Jun, Yang Rui, Cao Yi, Zhu Xiaotong, Zhang Hai, Chen Yu-Xia, Shi Yuguang, Shi Guang-Xia, Zou Dajin, Cao Xuetao, Grusby Michael J, Xie Zhifang, Zhang Weiping J

机构信息

Department of Pathophysiology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.

Department of Pathophysiology, Dalian Medical University, 9 West Section, Lvshun South Road, Dalian, 116044, China.

出版信息

Nat Commun. 2016 Apr 15;7:11121. doi: 10.1038/ncomms11121.

DOI:10.1038/ncomms11121
PMID:27079169
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4835541/
Abstract

The anterior pituitary harbours five distinct hormone-producing cell types, and their cellular differentiation is a highly regulated and coordinated process. Here we show that ZBTB20 is essential for anterior pituitary development and lactotrope specification in mice. In anterior pituitary, ZBTB20 is highly expressed by all the mature endocrine cell types, and to some less extent by somatolactotropes, the precursors of prolactin (PRL)-producing lactotropes. Disruption of Zbtb20 leads to anterior pituitary hypoplasia, hypopituitary dwarfism and a complete loss of mature lactotropes. In ZBTB20-null mice, although lactotrope lineage commitment is normally initiated, somatolactotropes exhibit profound defects in lineage specification and expansion. Furthermore, endogenous ZBTB20 protein binds to Prl promoter, and its knockdown decreases PRL expression and secretion in a lactotrope cell line MMQ. In addition, ZBTB20 overexpression enhances the transcriptional activity of Prl promoter in vitro. In conclusion, our findings point to ZBTB20 as a critical regulator of anterior pituitary development and lactotrope specification.

摘要

腺垂体包含五种不同的激素产生细胞类型，它们的细胞分化是一个高度受调控且协调的过程。在此我们表明，ZBTB20对小鼠腺垂体发育和催乳素细胞特化至关重要。在腺垂体中，ZBTB20在所有成熟内分泌细胞类型中高表达，而在催乳素（PRL）产生细胞的前体——生长催乳素细胞中表达程度稍低。Zbtb20的缺失导致腺垂体发育不全、垂体性侏儒症以及成熟催乳素细胞完全缺失。在ZBTB20基因敲除小鼠中，尽管催乳素细胞系的定向分化通常已启动，但生长催乳素细胞在细胞系特化和扩增方面表现出严重缺陷。此外，内源性ZBTB20蛋白与Prl启动子结合，其敲低会降低催乳素细胞系MMQ中PRL的表达和分泌。另外，ZBTB20的过表达在体外增强了Prl启动子的转录活性。总之，我们的研究结果表明ZBTB20是腺垂体发育和催乳素细胞特化的关键调节因子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/17e41b9c7812/ncomms11121-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/dd99a8790450/ncomms11121-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/95300e060a69/ncomms11121-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/02a690c0c507/ncomms11121-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/3f2c5e66c414/ncomms11121-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/2ae320371fc4/ncomms11121-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/64d61344704a/ncomms11121-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/8dc66a659852/ncomms11121-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/17e41b9c7812/ncomms11121-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/dd99a8790450/ncomms11121-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/95300e060a69/ncomms11121-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/02a690c0c507/ncomms11121-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/3f2c5e66c414/ncomms11121-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/2ae320371fc4/ncomms11121-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/64d61344704a/ncomms11121-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/8dc66a659852/ncomms11121-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dfcf/4835541/17e41b9c7812/ncomms11121-f8.jpg

相似文献

1
ZBTB20 is required for anterior pituitary development and lactotrope specification.垂体前叶发育和催乳素细胞分化需要ZBTB20。
Nat Commun. 2016 Apr 15;7:11121. doi: 10.1038/ncomms11121.
2
ZBTB20 Regulates Prolactin Expression and Lactotrope Function in Adult Mice.ZBTB20调节成年小鼠催乳素的表达和促乳素细胞功能。
Endocrinology. 2022 Oct 23;163(12). doi: 10.1210/endocr/bqac181.
3
The Transcription Factor NR4A2 Plays an Essential Role in Driving Prolactin Expression in Female Pituitary Lactotropes.转录因子 NR4A2 在雌性垂体泌乳素细胞中驱动催乳素表达中发挥重要作用。
Endocrinology. 2020 May 1;161(5). doi: 10.1210/endocr/bqaa046.
4
Involvement of insulin-like growth factor-I for the regulation of prolactin synthesis by estrogen and postnatal proliferation of lactotrophs in the mouse anterior pituitary.胰岛素样生长因子-I 在雌激素调节催乳素合成和产后小鼠垂体前叶泌乳细胞增殖中的作用。
Cell Tissue Res. 2010 Apr;340(1):147-58. doi: 10.1007/s00441-010-0937-0. Epub 2010 Mar 3.
5
Regenerative capacity of the adult pituitary: multiple mechanisms of lactotrope restoration after transgenic ablation.成年垂体的再生能力：转基因消融后催乳素细胞恢复的多种机制。
Stem Cells Dev. 2012 Dec 10;21(18):3245-57. doi: 10.1089/scd.2012.0290. Epub 2012 Oct 16.
6
Estrogen inhibits D2S receptor-regulated Gi3 and Gs protein interactions to stimulate prolactin production and cell proliferation in lactotropic cells.雌激素抑制 D2S 受体调节的 Gi3 和 Gs 蛋白相互作用，从而刺激催乳素细胞中的催乳素产生和细胞增殖。
J Endocrinol. 2012 Jul;214(1):67-78. doi: 10.1530/JOE-12-0125. Epub 2012 May 9.
7
Transcriptome Analyses of Female Somatotropes and Lactotropes Reveal Novel Regulators of Cell Identity in the Pituitary.转录组分析雌性生长激素细胞和催乳素细胞揭示了垂体细胞身份的新调控因子。
Endocrinology. 2018 Dec 1;159(12):3965-3980. doi: 10.1210/en.2018-00587.
8
Effect of transcription factor ZBTB20 on mouse pituitary development.转录因子ZBTB20对小鼠垂体发育的影响。
Genet Mol Res. 2015 Dec 21;14(4):17622-9. doi: 10.4238/2015.December.21.35.
9
Prolactin induces apoptosis of lactotropes in female rodents.催乳素诱导雌性啮齿动物的催乳细胞凋亡。
PLoS One. 2014 May 23;9(5):e97383. doi: 10.1371/journal.pone.0097383. eCollection 2014.
10
Differentiation of lactotrope precursor GHFT cells in response to fibroblast growth factor-2.催乳素细胞前体GHFT细胞对成纤维细胞生长因子-2的分化。
J Biol Chem. 2000 Jul 14;275(28):21653-60. doi: 10.1074/jbc.M002129200.

引用本文的文献

1
Diagnostic Challenges of Short Stature and Growth Hormone Insufficiency Across Different Genetic Etiologies.不同遗传病因导致的身材矮小和生长激素缺乏症的诊断挑战
Biomedicines. 2025 Aug 8;13(8):1937. doi: 10.3390/biomedicines13081937.
2
Regulation of carcinogenesis through multiple zinc fingers in ZBTB20.通过ZBTB20中的多个锌指对致癌作用的调控。
RSC Chem Biol. 2025 Jun 9. doi: 10.1039/d5cb00088b.
3
Single-cell multi-dimensional data analysis decodes RNF19A-mediated drug resistance in rheumatoid arthritis fibroblast-like synoviocytes: mechanisms and biological insights.

本文引用的文献

1
ZBTB20 is a sequence-specific transcriptional repressor of alpha-fetoprotein gene.锌指蛋白20是甲胎蛋白基因的序列特异性转录抑制因子。
Sci Rep. 2015 Jul 15;5:11979. doi: 10.1038/srep11979.
2
Zbtb20 regulates the terminal differentiation of hypertrophic chondrocytes via repression of Sox9.锌指蛋白20（Zbtb20）通过抑制Sox9调控肥大软骨细胞的终末分化。
Development. 2015 Jan 15;142(2):385-93. doi: 10.1242/dev.108530.
3
ZBTB20 regulates nociception and pain sensation by modulating TRP channel expression in nociceptive sensory neurons.
单细胞多维数据分析揭示类风湿关节炎成纤维样滑膜细胞中RNF19A介导的耐药性：机制与生物学见解
Cell Mol Life Sci. 2025 Apr 28;82(1):180. doi: 10.1007/s00018-025-05707-8.
4
Systematic Analysis of the Relationship Between Elevated Zinc and Epilepsy.锌水平升高与癫痫的关系的系统分析。
J Mol Neurosci. 2024 Apr 6;74(2):39. doi: 10.1007/s12031-024-02213-7.
5
Zinc Finger and BTB Domain-Containing 20: A Newly Emerging Player in Pathogenesis and Development of Human Cancers.锌指和 BTB 结构域蛋白 20：人类癌症发病机制和发展中的新出现的参与者。
Biomolecules. 2024 Feb 4;14(2):192. doi: 10.3390/biom14020192.
6
Fructose overconsumption impairs hepatic manganese homeostasis and ammonia disposal.果糖摄入过量会损害肝脏内的锰稳态和氨处理。
Nat Commun. 2023 Dec 1;14(1):7934. doi: 10.1038/s41467-023-43609-0.
7
Transcription Factor Zbtb20 as a Regulator of Malignancy and Its Practical Applications.转录因子 Zbtb20 作为恶性肿瘤的调节剂及其实际应用。
Int J Mol Sci. 2023 Sep 6;24(18):13763. doi: 10.3390/ijms241813763.
8
Glial Populations in the Human Brain Following Ischemic Injury.缺血性损伤后人脑的神经胶质细胞群
Biomedicines. 2023 Aug 22;11(9):2332. doi: 10.3390/biomedicines11092332.
9
ZBTB20 is essential for cochlear maturation and hearing in mice.ZBTB20 对于小鼠耳蜗成熟和听力至关重要。
Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2220867120. doi: 10.1073/pnas.2220867120. Epub 2023 Jun 6.
10
Transcriptomic Profiles of Normal Pituitary Cells and Pituitary Neuroendocrine Tumor Cells.正常垂体细胞和垂体神经内分泌肿瘤细胞的转录组图谱
Cancers (Basel). 2022 Dec 24;15(1):110. doi: 10.3390/cancers15010110.
ZBTB20 通过调节伤害性感觉神经元中瞬时受体电位（TRP）通道的表达来调控伤害感受和痛觉。
Nat Commun. 2014 Nov 5;5:4984. doi: 10.1038/ncomms5984.
4
Selective disruption of dopamine D2 receptors in pituitary lactotropes increases body weight and adiposity in female mice.选择性破坏催乳素细胞中的多巴胺 D2 受体可增加雌性小鼠的体重和肥胖度。
Endocrinology. 2014 Mar;155(3):829-39. doi: 10.1210/en.2013-1707. Epub 2013 Jan 1.
5
Zinc finger protein ZBTB20 promotes Toll-like receptor-triggered innate immune responses by repressing IκBα gene transcription.锌指蛋白 ZBTB20 通过抑制 IκBα 基因转录促进 Toll 样受体触发的固有免疫反应。
Proc Natl Acad Sci U S A. 2013 Jul 2;110(27):11097-102. doi: 10.1073/pnas.1301257110. Epub 2013 Jun 17.
6
Pituitary development: a complex, temporal regulated process dependent on specific transcriptional factors.垂体发育：一个复杂的、受时间调控的过程，依赖于特定的转录因子。
J Endocrinol. 2012 Nov;215(2):239-45. doi: 10.1530/JOE-12-0229. Epub 2012 Aug 7.
7
The adult pituitary shows stem/progenitor cell activation in response to injury and is capable of regeneration.成人垂体在受到损伤时会激活干细胞/祖细胞，从而具有再生能力。
Endocrinology. 2012 Jul;153(7):3224-35. doi: 10.1210/en.2012-1152. Epub 2012 Apr 19.
8
The zinc finger protein ZBTB20 regulates transcription of fructose-1,6-bisphosphatase 1 and β cell function in mice.锌指蛋白 ZBTB20 调控果糖-1,6-二磷酸酶 1 的转录和β细胞功能在小鼠体内的作用。
Gastroenterology. 2012 Jun;142(7):1571-1580.e6. doi: 10.1053/j.gastro.2012.02.043. Epub 2012 Feb 25.
9
Self-formation of functional adenohypophysis in three-dimensional culture.三维培养中功能性腺垂体的自形成。
Nature. 2011 Nov 9;480(7375):57-62. doi: 10.1038/nature10637.
10
Nestin-Cre mice are affected by hypopituitarism, which is not due to significant activity of the transgene in the pituitary gland.Nestin-Cre 小鼠受到垂体功能减退症的影响，但这并不是由于转基因在垂体中的显著活性所致。
PLoS One. 2010 Jul 6;5(7):e11443. doi: 10.1371/journal.pone.0011443.