Pislyagin E A, Manzhulo I V, Dmitrenok P S, Aminin D L
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
A.V.Zhirmunsky Institute of Marine Biology, Far Eastern Branch of Russian Academy of Science, Vladivostok, Russia; School of Biomedicine, Far Eastern Federal University, Vladivostok, Russia.
Acta Histochem. 2016 May;118(4):387-92. doi: 10.1016/j.acthis.2016.03.009. Epub 2016 Apr 11.
The immunomodulatory effect of triterpene glycoside cucumarioside A2-2 (CA2-2), isolated from the Far Eastern sea cucumber Cucumaria japonica, on the mouse spleen was investigated in comparison with lipopolysaccharide (LPS). It has been shown that the intraperitoneal (i.p.) glycoside administration did not influence on splenic weights, while the statistically significant increase in splenic weight was observed after LPS administration. Changes in the ratio of red to white pulp after CA2-2 or LPS administration were observed. The proportion of splenic white pulp after glycoside or LPS administration increased by up to 34% and 36%, respectively. A detailed study of the distribution of the РСNA (Proliferating Cell Nuclear Antigen) marker showed that the proliferative activity in the white pulp under CA2-2 and LPS influence increased 2.07 and 2.24 times, respectively. The localization of PCNA-positive nuclei in the white pulp region, as well as their dimensional characteristics, suggests that a large proportion of the proliferating cell population consisted of B cells. The mass spectrometry profiles of spleen peptide/protein homogenate were obtained using the MALDI-TOF-MS (Matrix -Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry) approach. It was found that i.p. stimulation of animals with CA2-2 or LPS leads to marked changes in the intensity of revealed characteristic peaks of peptides/proteins after exposure to immunostimulants.
研究了从远东海参日本刺参中分离出的三萜糖苷海参皂苷A2-2（CA2-2）与脂多糖（LPS）相比对小鼠脾脏的免疫调节作用。结果表明，腹腔注射该糖苷对脾脏重量没有影响，而注射LPS后脾脏重量有统计学意义的增加。观察到CA2-2或LPS给药后红髓与白髓比例的变化。糖苷或LPS给药后脾白髓比例分别增加了34%和36%。对增殖细胞核抗原（PCNA）标记物分布的详细研究表明，在CA2-2和LPS影响下白髓中的增殖活性分别增加了2.07倍和2.24倍。PCNA阳性细胞核在白髓区域的定位及其尺寸特征表明，增殖细胞群体中很大一部分是B细胞。使用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）方法获得了脾脏肽/蛋白质匀浆的质谱图。发现用CA2-2或LPS腹腔刺激动物会导致在暴露于免疫刺激剂后所揭示的肽/蛋白质特征峰强度发生显著变化。
