Akedo H, Shinkai K, Mukai M, Komatsu K
Department of Tumor Biochemistry, Center for Adult Diseases, Osaka, Japan.
Invasion Metastasis. 1989;9(2):134-48.
A culture model for invasion of rat mesothelial cell layer by rat ascites hepatoma cells has been developed. By using this quantitative model, the preculture with macrophages (0.1 less than macrophage/tumor cell less than 1.0) was found to enhance both the in vitro and in vivo invasive potentials of the tumor cells. This potentiation appears to be mediated partly by oxygen radicals generated by the cocultured macrophages. The in vitro invasive capacity was also augmented by pretreating the tumor cells with TGF-beta or with activated platelets. A factor with anti-invasive potential (IIF) was extracted from rat liver. It inhibited the directed migration but not the growth of the tumor cells and was effective on their in vivo invasion and metastasis, as well.
已建立一种大鼠腹水肝癌细胞侵袭大鼠间皮细胞层的培养模型。通过使用该定量模型，发现用巨噬细胞进行预培养（巨噬细胞/肿瘤细胞比例为0.1至1.0）可增强肿瘤细胞的体外和体内侵袭潜能。这种增强作用似乎部分由共培养的巨噬细胞产生的氧自由基介导。用转化生长因子-β或活化血小板预处理肿瘤细胞也可增强其体外侵袭能力。从大鼠肝脏中提取出一种具有抗侵袭潜能的因子（IIF）。它抑制肿瘤细胞的定向迁移但不抑制其生长，并且对肿瘤细胞的体内侵袭和转移也有效。
