Mukai M, Shinkai K, Yoshioka K, Imamura F, Akedo H
Department of Tumor Biochemistry, Research Institute Center for Adult Diseases, Osaka, Japan.
Hum Cell. 1993 Sep;6(3):194-8.
A culture model for invasion of rat mesothelial cell layer by rat ascites hepatoma cells has been developed. By using this quantitative model, we have recently found that the invasiveness of tumor cells is not only genetically determined but is greatly influenced by their interactions with host cells and host mediators. The preculture with macrophages was found to enhance both the in vitro and in vivo invasive potentials of the tumor cells. This potentiation appears to be mediated partly by oxygen radicals generated by the cocultured macrophages. The in vitro invasive capacity was also augmented by pretreating the tumor cells with TGF-beta, or with activated platelets. In the in vitro invasion assay system, tumor cells did not invade against cultured mesothelial cell monolayers without fetal calf serum. Serum could be completely substituted by oleoyl-lysophosphatidic acid (LPA) or bacterial phospholipase D (PLD), suggesting a possible participation of particular signaling cascade, PLD-LPA(PA) system, in the invasion of certain tumor cells.
已建立大鼠腹水肝癌细胞侵袭大鼠间皮细胞层的培养模型。通过使用这个定量模型，我们最近发现肿瘤细胞的侵袭性不仅由基因决定，还受到它们与宿主细胞和宿主介质相互作用的极大影响。发现与巨噬细胞预培养可增强肿瘤细胞的体外和体内侵袭潜能。这种增强作用似乎部分由共培养的巨噬细胞产生的氧自由基介导。用转化生长因子-β（TGF-β）或活化血小板预处理肿瘤细胞也可增强其体外侵袭能力。在体外侵袭试验系统中，没有胎牛血清时肿瘤细胞不会侵袭培养的间皮细胞单层。血清可被油酰溶血磷脂酸（LPA）或细菌磷脂酶D（PLD）完全替代，这表明特定信号级联反应，即PLD-LPA（PA）系统，可能参与某些肿瘤细胞的侵袭过程。
